Typical Testing Data/Standard Curve (for reference only)
Rabbit D5R/DRD5 ELISA Kit | D5R/DRD5 elisa kit
Rabbit D5R/DRD5 (Dopamine Receptor D5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to D5R/DRD5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for D5R/DRD5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain D5R/DRD5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of D5R/DRD5. You can calculate the concentration of D5R/DRD5 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Cellular Component: membrane; cell soma; integral to plasma membrane; brush border membrane; axon; dendrite; dendritic spine; integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; signal transducer activity; dopamine receptor activity; dopamine binding; G-protein alpha-subunit binding; dopamine D1 receptor-like receptor activity; drug binding
Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; wound healing; response to amphetamine; mating behavior; transmission of nerve impulse; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; response to cocaine; signal transduction; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; sensitization; positive regulation of cAMP biosynthetic process; dopamine receptor signaling pathway; negative regulation of protein kinase activity; visual learning; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; negative regulation of NAD(P)H oxidase activity; negative regulation of cell migration; associative learning; dopamine receptor, adenylate cyclase activating pathway
Research Articles on D5R/DRD5
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Product Notes
The Rabbit D5R/DRD5 drd5 (Catalog #AAA2510683) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510683 ELISA Kit recognizes Rabbit D5R/DRD5. It is sometimes possible for the material contained within the vial of "D5R/DRD5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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