Rabbit anti-Human ANP32E Polyclonal Antibody | anti-ANP32E antibody

ANP32E Antibody (N-term)

Cat. Num. AAA9215748

• Gene Names: ANP32E; LANPL; LANP-L
• Reactivity: Human
• Applications: Western Blot
• Synonyms: ANP32E; Polyclonal Antibody; ANP32E Antibody (N-term); Purified Rabbit Polyclonal Antibody (Pab); Acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-like protein; LANP-L; anti-ANP32E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This ANP32E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 31-64 amino acids from the N-terminal region of human ANP32E.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 227

• Applicable Applications for anti-ANP32E antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-ANP32E Antibody (N-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: human skeletal muscle lysatesLane 3: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ANP32E antibody

Histone chaperone that specifically mediates the genome- wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.

Product Categories/Family for anti-ANP32E antibody

Cell Biology; Metabolism; Neuroscience

References

Jiang M.,et al.Cytogenet. Genome Res. 97:68-71(2002). Ota T.,et al.Nat. Genet. 36:40-45(2004). Bechtel S.,et al.BMC Genomics 8:399-399(2007). Gregory S.G.,et al.Nature 441:315-321(2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 210147569
• NCBI GeneID: 81611
• NCBI Accession #: NP_001129950.1
• NCBI GenBank Nucleotide #: NM_001136478.3
• UniProt Accession #: Q9BTT0; Q5TB18; Q5TB20; Q8N1S4; Q8WWW9; B4E0I6; E9PEA6
• Molecular Weight: 30692
• NCBI Official Full Name: acidic leucine-rich nuclear phosphoprotein 32 family member E isoform 2
• NCBI Official Synonym Full Names: acidic nuclear phosphoprotein 32 family member E
• NCBI Official Symbol: ANP32E
• NCBI Official Synonym Symbols: LANPL; LANP-L
• NCBI Protein Information: acidic leucine-rich nuclear phosphoprotein 32 family member E
• UniProt Protein Name: Acidic leucine-rich nuclear phosphoprotein 32 family member E
• Protein Family: Acidic leucine-rich nuclear phosphoprotein 32 family
• UniProt Gene Name: ANP32E
• UniProt Synonym Gene Names: LANP-L
• UniProt Entry Name: AN32E_HUMAN

NCBI Description

LANP-L: Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity. Belongs to the ANP32 family.

Protein type: Motility/polarity/chemotaxis; Inhibitor; Protein phosphatase, regulatory subunit; Vesicle

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasmic membrane-bound vesicle; nucleus

Molecular Function: phosphatase inhibitor activity; histone binding

Biological Process: negative regulation of catalytic activity; histone exchange

Uniprot Description

LANP-L: Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity. Belongs to the ANP32 family.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis; Vesicle; Inhibitor

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasmic membrane-bound vesicle; nucleus

Molecular Function: histone binding; phosphatase inhibitor activity

Biological Process: histone exchange; negative regulation of catalytic activity