UBA52 sirna

UBA52 siRNA (Mouse)

Cat. Num. AAA8238031

• Gene Names: Uba52; Ubb; Ubc; Gm1863; Rps27a; D8Ertd21e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBA52; UBA52 siRNA (Mouse); UBCEP2; Ubiquitin-60S ribosomal protein L40; Ubiquitin A-52 residue ribosomal protein fusion product 1; UBA52 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UBA52 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 128

• Applicable Applications for UBA52 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UBA52 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBA52 sirna

siRNA to inhibit UBA52 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9845265
• NCBI GeneID: 22186
• NCBI Accession #: NP_063936.1
• NCBI GenBank Nucleotide #: NM_019883.3
• UniProt Accession #: P62984; P02248; P02249; P02250; P62991; Q29120; Q62317; Q64223; Q8VCH1; Q91887; Q91888; Q9CXY4
• Molecular Weight: 14,728 Da
• NCBI Official Full Name: ubiquitin-60S ribosomal protein L40
• NCBI Official Synonym Full Names: ubiquitin A-52 residue ribosomal protein fusion product 1
• NCBI Official Symbol: Uba52
• NCBI Official Synonym Symbols: Ubb; Ubc; Gm1863; Rps27a; D8Ertd21e
• NCBI Protein Information: ubiquitin-60S ribosomal protein L40
• UniProt Protein Name: Ubiquitin-60S ribosomal protein L40
• UniProt Gene Name: Uba52
• UniProt Synonym Gene Names: Ubcep2
• UniProt Entry Name: RL40_MOUSE

Uniprot Description

ubiquitin: a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. Only the 76 amino acids of monoubiquitin product are shown in this entry. At the protein level, it is not possible to determine which of the four genes a given ubiquitin chain was derived from. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Protein type: Ubiquitin-like modifier