Rabbit anti-Human ONECUT3 Polyclonal Antibody | anti-ONECUT3 antibody

ONECUT3, CT (ONECUT3, One cut domain family member 3, One cut homeobox 3, Transcription factor ONECUT-3) (Azide free) (HRP)

Cat. Num. AAA6327803

• Gene Names: ONECUT3; OC3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ONECUT3; Polyclonal Antibody; CT (ONECUT3; One cut domain family member 3; One cut homeobox 3; Transcription factor ONECUT-3) (Azide free) (HRP); Transcription factor ONECUT-3; anti-ONECUT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ONECUT3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ONECUT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392-419 amino acids from the C-terminal region of human ONECUT3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ONECUT3 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ONECUT3 antibody detected the ONECUT3 protein (arrow).)

Related Product Information for anti-ONECUT3 antibody

ONECUT3 is transcriptional activator. It binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).

Product Categories/Family for anti-ONECUT3 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Grimwood, J., et al. Nature 428(6982):529-535(2004). Vanhorenbeeck, V., et al. Biochem. Biophys. Res. Commun. 292(4):848-854(2002). Venter, J.C., et al. Science 291(5507):1304-1351(2001). Jacquemin, P., et al. J. Biol. Chem. 274(5):2665-2671(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 122937424
• NCBI GeneID: 390874
• NCBI Accession #: NP_001073957.1
• NCBI GenBank Nucleotide #: NM_001080488.1
• UniProt Accession #: O60422; A8MZM7
• Molecular Weight: 50,037 Da
• NCBI Official Full Name: one cut domain family member 3
• NCBI Official Synonym Full Names: one cut homeobox 3
• NCBI Official Symbol: ONECUT3
• NCBI Official Synonym Symbols: OC3
• NCBI Protein Information: one cut domain family member 3; OC-3; transcription factor ONECUT-3; one cut domain, family member 3
• UniProt Protein Name: One cut domain family member 3
• Protein Family: One cut domain family
• UniProt Gene Name: ONECUT3
• UniProt Synonym Gene Names: OC-3

Uniprot Description

ONECUT3: Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: cell differentiation; system development