NCKIPSD sirna

NCKIPSD siRNA (Human)

Cat. Num. AAA8236896

• Gene Names: NCKIPSD; DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NCKIPSD; NCKIPSD siRNA (Human); AF3P21; SPIN90; NCK-interacting protein with SH3 domain; 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; VIP54; 90 kDa SH3 protein interacting with Nck; AF3p21; Dia-interacting protein 1; DIP-1; Diaphanous protein-interacting protein; SH3 adapter protein SPIN90; WASP-interacting SH3-domain protein; WISH; Wiskott-Aldrich syndrome protein-interacting protein; NCKIPSD sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NCKIPSD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 596

• Applicable Applications for NCKIPSD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NCKIPSD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NCKIPSD sirna

siRNA to inhibit NCKIPSD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7705266
• NCBI GeneID: 51517
• NCBI Accession #: NP_057537.1
• NCBI GenBank Nucleotide #: NM_016453.3
• UniProt Accession #: Q9NZQ3; Q6GU34; Q6SPF3; Q8TC10; Q9UGM8; B4DFL5
• Molecular Weight: 64,196 Da
• NCBI Official Full Name: NCK-interacting protein with SH3 domain isoform 1
• NCBI Official Synonym Full Names: NCK interacting protein with SH3 domain
• NCBI Official Symbol: NCKIPSD
• NCBI Official Synonym Symbols: DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
• NCBI Protein Information: NCK-interacting protein with SH3 domain
• UniProt Protein Name: NCK-interacting protein with SH3 domain
• Protein Family: NCK-interacting protein with SH3 domain
• UniProt Gene Name: NCKIPSD
• UniProt Synonym Gene Names: AF3P21; SPIN90; VIP54; DIP-1; WISH
• UniProt Entry Name: SPN90_HUMAN

NCBI Description

The protein encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

NCKIPSD: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo. In vitro, stimulates N-WASP- induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis. A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: signalosome; intermediate filament; cytosol

Molecular Function: protein binding; cytoskeletal protein binding; SH3 domain binding

Biological Process: innate immune response; cytoskeleton organization and biogenesis; NLS-bearing substrate import into nucleus; signal transduction