APOA1 sirna

APOA1 siRNA (Mouse)

Cat. Num. AAA8236471

• Gene Names: Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOA1; APOA1 siRNA (Mouse); Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1; APOA1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: APOA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 264

• Applicable Applications for APOA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse APOA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOA1 sirna

siRNA to inhibit APOA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 160333304
• NCBI GeneID: 11806
• NCBI Accession #: NP_033822.2
• NCBI GenBank Nucleotide #: NM_009692.4
• UniProt Accession #: Q00623; O08855; O09042; Q8BPD5
• Molecular Weight: 30,616 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_MOUSE

NCBI Description

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Vesicle; Endoplasmic reticulum; Motility/polarity/chemotaxis; Lipid-binding; Secreted, signal peptide; Cell development/differentiation; Secreted

Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; extracellular region; cytoplasmic vesicle; nucleus; vesicle

Molecular Function: lipid transporter activity; identical protein binding; protein binding; enzyme binding; phospholipid transporter activity; lipase inhibitor activity; beta-amyloid binding; chemorepellent activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; apolipoprotein A-I receptor binding; high-density lipoprotein binding; lipid binding; apolipoprotein receptor binding

Biological Process: steroid metabolic process; blood vessel endothelial cell migration; negative chemotaxis; negative regulation of lipase activity; phosphatidylcholine metabolic process; negative regulation of interleukin-1 beta secretion; protein localization; sequestering of lipid; phospholipid efflux; regulation of cholesterol absorption; transport; negative regulation of hydrolase activity; phospholipid metabolic process; positive regulation of stress fiber formation; phospholipid homeostasis; integrin-mediated signaling pathway; cholesterol metabolic process; positive regulation of fatty acid biosynthetic process; regulation of protein amino acid phosphorylation; organ regeneration; regulation of Cdc42 protein signal transduction; cholesterol transport; protein stabilization; protein amino acid oxidation; negative regulation of heterotypic cell-cell adhesion; adrenal gland development; axon regeneration; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; cholesterol efflux; positive regulation of transferase activity; vitamin transport; lipid transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; glucocorticoid metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; lipoprotein biosynthetic process; endothelial cell proliferation; negative regulation of inflammatory response; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; phospholipid transport; lipid metabolic process