UBE2L3 sirna

UBE2L3 siRNA (Mouse)

Cat. Num. AAA8236325

• Gene Names: Ube2l3; UbcM4; Ubce7; C79827
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBE2L3; UBE2L3 siRNA (Mouse); UBCE7; Ubiquitin-conjugating enzyme E2 L3; UbcM4; Ubiquitin carrier protein L3; Ubiquitin-protein ligase L3; UBE2L3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UBE2L3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 154

• Applicable Applications for UBE2L3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UBE2L3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBE2L3 sirna

siRNA to inhibit UBE2L3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678481
• NCBI GeneID: 22195
• NCBI Accession #: NP_033482.1
• NCBI GenBank Nucleotide #: NM_009456.2
• UniProt Accession #: P68037; P51966; P70653; Q9HAV1
• Molecular Weight: 17,862 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 L3
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2L 3
• NCBI Official Symbol: Ube2l3
• NCBI Official Synonym Symbols: UbcM4; Ubce7; C79827
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 L3
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: Ube2l3
• UniProt Synonym Gene Names: Ubce7
• UniProt Entry Name: UB2L3_MOUSE

Uniprot Description

UBE2L3: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1. Ubiquitous, with highest expression in testis. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; Nuclear receptor co-regulator; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Cellular Component: cytoplasm; nucleus

Molecular Function: ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity

Biological Process: cell proliferation; protein polyubiquitination; regulation of transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination