Human Selenium Binding Protein 1 (SELENBP1) ELISA Kit | SELENBP1 elisa kit

Human Selenium Binding Protein 1 (SELENBP1) ELISA Kit

Cat. Num. AAA2706076

• Gene Names: SELENBP1; LPSB; SP56; hSBP; SBP56; HEL-S-134P
• Reactivity: Human
• Synonyms: Selenium Binding Protein 1 (SELENBP1); Human Selenium Binding Protein 1 (SELENBP1) ELISA Kit; LPSB; SP56; hSBP; hSP56; SBP56; 56 kDa selenium-binding protein; SELENBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Selenium Binding Protein 1 (SELENBP1).; No significant cross-reactivity or interference between Selenium Binding Protein 1 (SELENBP1) and analogues was observed.
• Sequence Length: 514

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selenium Binding Protein 1 (SELENBP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selenium Binding Protein 1 (SELENBP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELENBP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenium Binding Protein 1 (SELENBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selenium Binding Protein 1 (SELENBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenium Binding Protein 1 (SELENBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selenium Binding Protein 1 (SELENBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14290607
• NCBI GeneID: 8991
• UniProt Accession #: Q13228; Q49AQ8; Q96GX7; A6NML9; A6PVW9; B2RDR3; B4DKP6; B4E1F3
• Molecular Weight: 56,866 Da
• NCBI Official Full Name: Selenium binding protein 1
• NCBI Official Synonym Full Names: selenium binding protein 1
• NCBI Official Symbol: SELENBP1
• NCBI Official Synonym Symbols: LPSB; SP56; hSBP; SBP56; HEL-S-134P
• NCBI Protein Information: selenium-binding protein 1
• UniProt Protein Name: Selenium-binding protein 1
• Protein Family: Selenium-binding protein
• UniProt Gene Name: SELENBP1
• UniProt Synonym Gene Names: SBP; SBP56; SP56

NCBI Description

This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport ().