Erythropoietin Receptor (EPOR) Recombinant Protein | EPOR recombinant protein

Recombinant Erythropoietin Receptor (EPOR)

Cat. Num. AAA2011203

• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Erythropoietin Receptor (EPOR); Recombinant Erythropoietin Receptor (EPOR); EPOR recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Concentration: Original Concentration: 200 ug/mL (varies by lot)

• Applicable Applications for EPOR recombinant protein: Positive Control; Immunogen; SDS-PAGE; WB.; ; (May be suitable for use in other assays to be determined by the end user.)
• Organism Species: Rattus norvegicus (Rat)
• Source: Prokaryotic expression
• Residues: Leu314~Tyr467
• Tags: N-terminal His Tag
• Subcellular Location: Membrane
• Traits: Freeze-dried powder
• Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Predicted isoelectric point: 4.6
• Predicted Molecular Mass: 18.2kDa
• Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 2-8ºC for one month.; Aliquot and store at -80ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 729431
• NCBI GeneID: 24336
• UniProt Accession #: Q07303
• NCBI Official Full Name: Erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: Epor
• NCBI Protein Information: erythropoietin receptor; EPO-R
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: Epor
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_RAT

NCBI Description

binds erythropoietin and induces an increase in cytosolic calcium ion concentration [RGD, Feb 2006]

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: integral to membrane

Molecular Function: identical protein binding; erythropoietin receptor activity

Biological Process: elevation of cytosolic calcium ion concentration; heart development; negative regulation of neuron apoptosis; brain development; decidualization; signal transduction