NUP98 sirna

NUP98 siRNA (Human)

Cat. Num. AAA8232201

• Gene Names: NUP98; ADIR2; NUP96; NUP196
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUP98; NUP98 siRNA (Human); ADAR2; Nuclear pore complex protein Nup98-Nup96; NUP98 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NUP98 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 937

• Applicable Applications for NUP98 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NUP98 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUP98 sirna

siRNA to inhibit NUP98 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 56549643
• NCBI GeneID: 4928
• NCBI Accession #: NP_005378.4
• NCBI GenBank Nucleotide #: NM_005387.5
• UniProt Accession #: P52948; Q8IUT2; Q8WYB0; Q96E54; Q9H3Q4; Q9NT02; Q9UF57; Q9UHX0; Q9Y6J4; Q9Y6J5
• Molecular Weight: 186,268 Da
• NCBI Official Full Name: nuclear pore complex protein Nup98-Nup96 isoform 3
• NCBI Official Synonym Full Names: nucleoporin 98kDa
• NCBI Official Symbol: NUP98
• NCBI Official Synonym Symbols: ADIR2; NUP96; NUP196
• NCBI Protein Information: nuclear pore complex protein Nup98-Nup96
• UniProt Protein Name: Nuclear pore complex protein Nup98-Nup96
• UniProt Gene Name: NUP98
• UniProt Synonym Gene Names: ADAR2; Nup98; Nup96
• UniProt Entry Name: NUP98_HUMAN

NCBI Description

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, May 2010]

Uniprot Description

NUP98: Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. May interact with RAE1. Interacts with vesicular stomatitis virus protein M. Belongs to the nucleoporin GLFG family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nucleoporin

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: kinetochore; nucleoplasm; nuclear membrane; nuclear pore; nuclear envelope; cytosol; nuclear inclusion body

Molecular Function: protein binding; structural constituent of nuclear pore; transporter activity; peptide binding

Biological Process: nuclear pore complex assembly; mRNA transport; viral reproduction; protein import into nucleus, docking; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; nucleocytoplasmic transport; viral infectious cycle; glucose transport; nuclear pore organization and biogenesis; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; DNA replication; transmembrane transport