CORO1A sirna

CORO1A siRNA (Mouse)

Cat. Num. AAA8230187

• Gene Names: Coro1a; p57; Lmb3; TACO; Clabp
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CORO1A; CORO1A siRNA (Mouse); CORO1; Coronin-1A; Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO; CORO1A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CORO1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 461

• Applicable Applications for CORO1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CORO1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CORO1A sirna

siRNA to inhibit CORO1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 672890573
• NCBI GeneID: 12721
• NCBI Accession #: NP_001288303.1
• NCBI GenBank Nucleotide #: NM_001301374.1
• UniProt Accession #: O89053; Q7TMU0; Q9R1Y8; Q9R288
• Molecular Weight: 50,989 Da
• NCBI Official Full Name: coronin-1A isoform 1
• NCBI Official Synonym Full Names: coronin, actin binding protein 1A
• NCBI Official Symbol: Coro1a
• NCBI Official Synonym Symbols: p57; Lmb3; TACO; Clabp
• NCBI Protein Information: coronin-1A
• UniProt Protein Name: Coronin-1A
• Protein Family: Coronin
• UniProt Gene Name: Coro1a
• UniProt Synonym Gene Names: Coro1; Clipin-A; TACO
• UniProt Entry Name: COR1A_MOUSE

NCBI Description

This gene encodes a member of the WD repeat coronin family. The encoded protein may bind actin and interact with microtubules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: cortical actin cytoskeleton; protein complex; cell; leading edge; early endosome; immunological synapse; actin filament; intercellular junction; phagocytic vesicle; actin cytoskeleton; cytoskeleton; membrane; axon; lamellipodium; cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; phagocytic cup

Molecular Function: protein C-terminus binding; actin filament binding; identical protein binding; protein binding; protein homodimerization activity; cytoskeletal protein binding; phosphoinositide 3-kinase binding; actin binding

Biological Process: negative regulation of vesicle fusion; cell migration; leukocyte chemotaxis; regulation of actin polymerization and/or depolymerization; actin filament organization; cell-substrate adhesion; phagocytosis; regulation of release of sequestered calcium ion into cytosol; uropod organization and biogenesis; phagolysosome formation; regulation of cell shape; regulation of actin filament polymerization; positive chemotaxis; homeostasis of number of cells within a tissue; calcium ion transport; negative regulation of actin nucleation; response to cytokine stimulus; T cell homeostasis; positive regulation of T cell proliferation; negative regulation of neuron apoptosis; positive regulation of T cell activation; actin cytoskeleton organization and biogenesis; positive regulation of cell migration