Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
Rabbit TIMP4 Polyclonal Antibody | anti-TIMP4 antibody
TIMP4 Antibody
Predicted: Bovine, Horse, Rabbit
Predicted: Bovine, Horse, Rabbit
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA (Peptide): 1:20000-1:40000
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
Immunohistochemmistry (IHC)
(At 1/50 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/50 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, mouse muscle , using TIMP4 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, mouse muscle , using TIMP4 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various celllines,using TIMP4 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various celllines,using TIMP4 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using TIMP4 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using TIMP4 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using TIMP4 Antibody. Lane 1: B16F10, treated with blocking peptide; Lane 2: B16F10; Lane 3: Myeloma cells.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using TIMP4 Antibody. Lane 1: B16F10, treated with blocking peptide; Lane 2: B16F10; Lane 3: Myeloma cells.)
Similarity: Belongs to the protease inhibitor I35 (TIMP) family.
NCBI and Uniprot Product Information
Predicted: 26 kDa
NCBI Description
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq, Jul 2008]
Uniprot Description
TIMP4: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3p25
Cellular Component: extracellular space; proteinaceous extracellular matrix; sarcomere
Molecular Function: metalloendopeptidase inhibitor activity; protease binding; metal ion binding
Biological Process: response to drug; response to peptide hormone stimulus; negative regulation of metalloenzyme activity; ovulation cycle; central nervous system development; response to cytokine stimulus; response to hormone stimulus; response to lipopolysaccharide; negative regulation of membrane protein ectodomain proteolysis
Research Articles on TIMP4
Similar Products
Product Notes
The TIMP4 timp4 (Catalog #AAA9610371) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIMP4 Antibody reacts with Human, Mouse, Rat Predicted: Bovine, Horse, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's TIMP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the TIMP4 timp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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