NISCH sirna

NISCH siRNA (Human)

Cat. Num. AAA8229975

• Gene Names: NISCH; I-1; IR1; IRAS; hIRAS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NISCH; NISCH siRNA (Human); IRAS; KIAA0975; Nischarin; Imidazoline receptor 1; I-1; IR1; Imidazoline receptor antisera-selected protein; hIRAS; Imidazoline-1 receptor; I1R; Imidazoline-1 receptor candidate protein; I-1 receptor candidate protein; I1R candidate protein; NISCH sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NISCH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 583

• Applicable Applications for NISCH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NISCH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NISCH sirna

siRNA to inhibit NISCH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 443497968
• NCBI GeneID: 11188
• NCBI Accession #: NP_001263222.1
• NCBI GenBank Nucleotide #: NM_001276293.1
• UniProt Accession #: Q9Y2I1; Q6PGP3; Q6PIB4; Q7L8M3; Q7Z2X6; Q9UES6; Q9UEU4; Q9UFW3; C9J245
• Molecular Weight: 56,867 Da
• NCBI Official Full Name: nischarin isoform 2
• NCBI Official Synonym Full Names: nischarin
• NCBI Official Symbol: NISCH
• NCBI Official Synonym Symbols: I-1; IR1; IRAS; hIRAS
• NCBI Protein Information: nischarin
• UniProt Protein Name: Nischarin
• Protein Family: Nischarin
• UniProt Gene Name: NISCH
• UniProt Synonym Gene Names: IRAS; KIAA0975; I-1; IR1; hIRAS; I1R; I-1 receptor candidate protein; I1R candidate protein
• UniProt Entry Name: NISCH_HUMAN

NCBI Description

This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]

Uniprot Description

NISCH: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: recycling endosome; membrane; early endosome; plasma membrane; cytosol

Molecular Function: integrin binding; identical protein binding; protein binding; amine receptor activity; phosphoinositide binding

Biological Process: G-protein coupled receptor protein signaling pathway; norepinephrine secretion; apoptosis; regulation of blood pressure; glucose metabolic process; Rac protein signal transduction; actin cytoskeleton organization and biogenesis; negative regulation of cell migration; regulation of synaptic transmission, GABAergic