Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane protein 176A (TMEM176A) Standard Curve.)
Rat Transmembrane Protein 176A (TMEM176A) ELISA Kit | TMEM176A elisa kit
Rat Transmembrane Protein 176A (TMEM176A) ELISA Kit
Gene Names
Tmem176a; RGD1310725; 0610011i04rik
Reactivity
Rat
Synonyms
Transmembrane Protein 176A (TMEM176A); Rat Transmembrane Protein 176A (TMEM176A) ELISA Kit; TMEM176A; GS188; HCA112; hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188; TMEM176A elisa kit
Reactivity
Rat
Specificity
Rat Transmembrane protein 176A (TMEM176A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TMEM176A. No significant cross-reactivity or interference between Rat TMEM176A and analogues was observed.
Sequence Length
243
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane protein 176A (TMEM176A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane protein 176A (TMEM176A) Standard Curve.)
Related Product Information for TMEM176A elisa kit
Description: This Rat Transmembrane protein 176A (TMEM176A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM176A in samples. An antibody specific for TMEM176A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM176A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM176A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM176A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By serologic analysis of recombinant cDNA expression libraries (SEREX) from 4 hepatocellular carcinoma patients, Wang et al. (2002) cloned HCA112. The deduced protein contains 235 amino acids. RT-PCR detected HCA112 expression in human kidney, lung, and pancreas, but not in any other tissue examined. Nakajima et al. (2002) cloned the mouse homolog of HCA112, Gs188. The deduced 244-amino acid protein has a calculated molecular mass of 26.6 kD and contains 4 putative hydrophobic domains. Northern blot analysis detected Gs188 expression in mouse kidney, lung, and spleen; little to no expression was detected in other tissues examined.
Background: By serologic analysis of recombinant cDNA expression libraries (SEREX) from 4 hepatocellular carcinoma patients, Wang et al. (2002) cloned HCA112. The deduced protein contains 235 amino acids. RT-PCR detected HCA112 expression in human kidney, lung, and pancreas, but not in any other tissue examined. Nakajima et al. (2002) cloned the mouse homolog of HCA112, Gs188. The deduced 244-amino acid protein has a calculated molecular mass of 26.6 kD and contains 4 putative hydrophobic domains. Northern blot analysis detected Gs188 expression in mouse kidney, lung, and spleen; little to no expression was detected in other tissues examined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transmembrane protein 176A
NCBI Official Synonym Full Names
transmembrane protein 176A
NCBI Official Symbol
Tmem176a
NCBI Official Synonym Symbols
RGD1310725; 0610011i04rik
NCBI Protein Information
transmembrane protein 176A
UniProt Protein Name
Transmembrane protein 176A
Protein Family
UniProt Gene Name
Tmem176a
UniProt Entry Name
T176A_RAT
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Product Notes
The Rat TMEM176A tmem176a (Catalog #AAA9711702) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711702 ELISA Kit recognizes Rat TMEM176A. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 176A (TMEM176A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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