Human Nischarin (NISCH) ELISA Kit | NISCH elisa kit

Human Nischarin (NISCH) ELISA Kit

Cat. Num. AAA454295

• Gene Names: NISCH; I-1; IR1; IRAS; hIRAS
• Reactivity: Human
• Synonyms: Nischarin (NISCH); Human Nischarin (NISCH) ELISA Kit; NISCH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NISCH. No significant cross-reactivity or interference between NISCH and analogues was observed.
• Sequence Length: 583

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NISCH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NISCH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NISCH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NISCH in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NISCH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NISCH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NISCH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NISCH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 443497968
• NCBI GeneID: 11188
• NCBI Accession #: NP_001263222.1
• NCBI GenBank Nucleotide #: NM_001276293.1
• UniProt Accession #: Q9Y2I1; Q6PGP3; Q6PIB4; Q7L8M3; Q7Z2X6; Q9UES6; Q9UEU4; Q9UFW3; C9J245
• Molecular Weight: 56,867 Da
• NCBI Official Full Name: nischarin isoform 2
• NCBI Official Synonym Full Names: nischarin
• NCBI Official Symbol: NISCH
• NCBI Official Synonym Symbols: I-1; IR1; IRAS; hIRAS
• NCBI Protein Information: nischarin
• UniProt Protein Name: Nischarin
• Protein Family: Nischarin
• UniProt Gene Name: NISCH
• UniProt Synonym Gene Names: IRAS; KIAA0975; I-1; IR1; hIRAS; I1R; I-1 receptor candidate protein; I1R candidate protein

NCBI Description

This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]

Uniprot Description

Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (). Acts as a modulator of Rac-regulated signal transduction pathways (). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (). Inhibits lamellipodia formation, when overexpressed (). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.