Rabbit anti-Human, Mouse NISCH Polyclonal Antibody | anti-NISCH antibody

NISCH Polyclonal Antibody

Cat. Num. AAA9434477

• Gene Names: NISCH; I-1; IR1; IRAS; hIRAS
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: NISCH; Polyclonal Antibody; NISCH Polyclonal Antibody; I-1; IR1; IRAS; hIRAS; nischarin; anti-NISCH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, pH7.4

• Applicable Applications for anti-NISCH antibody: Western Blot (WB)
• Application Notes: WB: 1:200-1:2000
• Immunogen: Recombinant fusion protein of human NISCH (NP_009115.2).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NISCH antibody.)

Related Product Information for anti-NISCH antibody

This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor.

Product Categories/Family for anti-NISCH antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 66472382
• NCBI GeneID: 11188
• NCBI Accession #: NP_009115.2
• NCBI GenBank Nucleotide #: NM_007184.3
• UniProt Accession #: Q9Y2I1; Q6PGP3; Q6PIB4; Q7L8M3; Q7Z2X6; Q9UES6; Q9UEU4; Q9UFW3; C9J245
• Molecular Weight: 166,629 Da
• NCBI Official Full Name: nischarin isoform 1
• NCBI Official Synonym Full Names: nischarin
• NCBI Official Symbol: NISCH
• NCBI Official Synonym Symbols: I-1; IR1; IRAS; hIRAS
• NCBI Protein Information: nischarin; I1R candidate protein; imidazoline receptor 1; I-1 receptor candidate protein; imidazoline receptor antisera selected
• UniProt Protein Name: Nischarin
• Protein Family: Nischarin
• UniProt Gene Name: NISCH
• UniProt Synonym Gene Names: IRAS; KIAA0975; I-1; IR1; hIRAS; I1R; I-1 receptor candidate protein; I1R candidate protein

NCBI Description

This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]

Uniprot Description

NISCH: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytosol; early endosome; membrane; plasma membrane; recycling endosome

Molecular Function: identical protein binding; integrin binding; phosphatidylinositol binding; protein binding

Biological Process: actin cytoskeleton organization; apoptosis; glucose metabolic process; negative regulation of cell migration; norepinephrine secretion; Rac protein signal transduction; regulation of blood pressure; regulation of synaptic transmission, GABAergic