OBFC1 sirna
OBFC1 siRNA (Mouse)
Gene Names
Obfc1; AAF44; AAF-44; RPA-32; AI413458; 0610009H20Rik; 2310057J23Rik
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
OBFC1; OBFC1 siRNA (Mouse); STN1; CST complex subunit STN1; Alpha-accessory factor of 44 kDa; AAF-44; AAF44; Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog; OBFC1 sirna
Host
Synthetic
Reactivity
Mouse
Specificity
OBFC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
378
Applicable Applications for OBFC1 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OBFC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for OBFC1 sirna
siRNA to inhibit OBFC1 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,486 Da
NCBI Official Full Name
CST complex subunit STN1
NCBI Official Synonym Full Names
oligonucleotide/oligosaccharide-binding fold containing 1
NCBI Official Symbol
Obfc1
NCBI Official Synonym Symbols
AAF44; AAF-44; RPA-32; AI413458; 0610009H20Rik; 2310057J23Rik
NCBI Protein Information
CST complex subunit STN1
UniProt Protein Name
CST complex subunit STN1
Protein Family
UniProt Gene Name
Obfc1
UniProt Synonym Gene Names
Stn1; AAF-44; AAF44
UniProt Entry Name
STN1_MOUSE
Uniprot Description
OBFC1: Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Belongs to the STN1 family.
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; chromosome, telomeric region; nuclear chromosome, telomeric region; intracellular membrane-bound organelle; nucleolus; chromosome; nucleus
Molecular Function: protein binding; nucleic acid binding; single-stranded telomeric DNA binding; DNA binding; single-stranded DNA binding
Biological Process: telomere maintenance; positive regulation of DNA replication
Research Articles on OBFC1
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Product Notes
The OBFC1 obfc1 (Catalog #AAA8229785) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The OBFC1 siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OBFC1 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the OBFC1 obfc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OBFC1, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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