Rabbit anti-Human TBX5 Polyclonal Antibody | anti-TBX5 antibody

TBX5, NT (TBX5, T-box transcription factor TBX5) (FITC)

Cat. Num. AAA6354230

• Gene Names: TBX5; HOS
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBX5; Polyclonal Antibody; NT (TBX5; T-box transcription factor TBX5) (FITC); T-box transcription factor TBX5; anti-TBX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TBX5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TBX5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human TBX5, UniProt Accession #Q99593.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(All lanes: MBS647448 (1:2000) Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: HL-60 whole cell lysates Lysates/proteins (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (1:10,000) Predicted band size: ~58kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS647448. This demonstrates that MBS647448 detected the TBX5 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue usingMBS647448, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647448 for immunohistochemistry.)

Related Product Information for anti-TBX5 antibody

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

Product Categories/Family for anti-TBX5 antibody

Antibodies; Transcription Factors

References

1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Stirnimann, C.U., et al. J. Mol. Biol. 400(1):71-81(2010). 4. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 5. Hong, K.W., et al. J. Hum. Genet. 55(6):336-341(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 18201896
• NCBI GeneID: 6910
• UniProt Accession #: Q99593
• NCBI Official Synonym Full Names: T-box transcription factor 5
• NCBI Official Symbol: TBX5
• NCBI Official Synonym Symbols: HOS
• NCBI Protein Information: T-box transcription factor TBX5
• UniProt Protein Name: T-box transcription factor TBX5
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX5
• UniProt Synonym Gene Names: T-box protein 5
• UniProt Entry Name: TBX5_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX5: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX5 are the cause of Holt-Oram syndrome (HOS). HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; pericardium development; transcription initiation from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; pattern specification process; cardiac muscle cell differentiation; forelimb morphogenesis; negative regulation of cell proliferation; cell-cell signaling; apoptotic nuclear changes; negative regulation of cardiac muscle cell proliferation; positive regulation of cardioblast differentiation; morphogenesis of an epithelium; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell migration; lung development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation

Disease: Holt-oram Syndrome