ZFP57 sirna

ZFP57 siRNA (Human)

Cat. Num. AAA8227838

• Gene Names: ZFP57; TNDM1; ZNF698; C6orf40; bA145L22; bA145L22.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFP57; ZFP57 siRNA (Human); C6orf40; ZNF698; Zinc finger protein 57 homolog; Zfp-57; Zinc finger protein 698; ZFP57 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZFP57 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 536

• Applicable Applications for ZFP57 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZFP57 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZFP57 sirna

siRNA to inhibit ZFP57 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239582722
• NCBI GeneID: 346171
• NCBI Accession #: NP_001103279.2
• NCBI GenBank Nucleotide #: NM_001109809.2
• UniProt Accession #: Q9NU63; Q5SSB1; B0S894; B0V254; B2RXJ7
• Molecular Weight: 61,358 Da
• NCBI Official Full Name: zinc finger protein 57 homolog
• NCBI Official Synonym Full Names: ZFP57 zinc finger protein
• NCBI Official Symbol: ZFP57
• NCBI Official Synonym Symbols: TNDM1; ZNF698; C6orf40; bA145L22; bA145L22.2
• NCBI Protein Information: zinc finger protein 57 homolog
• UniProt Protein Name: Zinc finger protein 57 homolog
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZFP57
• UniProt Synonym Gene Names: C6orf40; ZNF698; Zfp-57
• UniProt Entry Name: ZFP57_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).[provided by RefSeq, Sep 2009]

Uniprot Description

ZFP57: Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions. Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Defects in ZFP57 are the cause of transient neonatal diabetes mellitus type 1 (TNDM1). Neonatal diabetes is a form of diabetes mellitus defined by the onset of mild-to- severe hyperglycemia within the first months of life. In about half of the neonates, diabetes is transient and resolves at a median age of 3 months, whereas the rest have a permanent form of diabetes. The major cause of TNDM1 is aberrant expression of imprinted genes at chromosome 6q24, associated in 20% of cases with DNA hypomethylation at the transient neonatal diabetes differentially methylated region (DMR), which lies within the imprinted promoter of the PLAGL1 gene. Over 50% of individuals with transient neonatal diabetes and hypomethylation at 6q24 also show mosaic DNA hypomethylation at other imprinted loci throughout the genome and a range of additional clinical features. Belongs to the krueppel C2H2-type zinc-finger protein family. ZFP57 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: nuclear heterochromatin

Molecular Function: DNA binding; metal ion binding

Biological Process: genetic imprinting; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; DNA methylation during embryonic development; peripheral nervous system development

Disease: Diabetes Mellitus, Transient Neonatal, 1