Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycerate kinase 1 (PGK1) Standard Curve.)
Mouse Phosphoglycerate Kinase 1 (PGK1) ELISA Kit | PGK1 elisa kit
Mouse Phosphoglycerate Kinase 1 (PGK1) ELISA Kit
Gene Names
Pgk1; Pgk-1
Reactivity
Mouse
Synonyms
Phosphoglycerate Kinase 1 (PGK1); Mouse Phosphoglycerate Kinase 1 (PGK1) ELISA Kit; PGK1; RP4-570L12.1; MGC117307; MGC142128; MGC8947; MIG10; PGKA; cell migration-inducing gene 10 protein; primer recognition protein 2; PGK1 elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphoglycerate kinase 1 (PGK1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PGK1. No significant cross-reactivity or interference between Mouse PGK1 and analogues was observed.
Sequence Length
417
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycerate kinase 1 (PGK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycerate kinase 1 (PGK1) Standard Curve.)
Related Product Information for PGK1 elisa kit
Description: This Mouse Phosphoglycerate kinase 1 (PGK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGK1 in samples. An antibody specific for PGK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The Phosphoglycerate Kinase 1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19.PGK1 is distinguished from testicular PGK2, which maps to chromosome 6p21. The deduced protein contains 417 amino acid residues. Southern blot analysis of human genomic DNAs showed a complex pattern of hybridizing fragments, 2 of which were non-X in origin. The results were interpreted as reflecting the existence of a small family of dispersed PGK or PGK-like genes.
Background: The Phosphoglycerate Kinase 1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19.PGK1 is distinguished from testicular PGK2, which maps to chromosome 6p21. The deduced protein contains 417 amino acid residues. Southern blot analysis of human genomic DNAs showed a complex pattern of hybridizing fragments, 2 of which were non-X in origin. The results were interpreted as reflecting the existence of a small family of dispersed PGK or PGK-like genes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoglycerate kinase 1
NCBI Official Synonym Full Names
phosphoglycerate kinase 1
NCBI Official Symbol
Pgk1
NCBI Official Synonym Symbols
Pgk-1
NCBI Protein Information
phosphoglycerate kinase 1
UniProt Protein Name
Phosphoglycerate kinase 1
Protein Family
UniProt Gene Name
Pgk1
UniProt Synonym Gene Names
Pgk-1
UniProt Entry Name
PGK1_MOUSE
NCBI Description
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
Uniprot Description
PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein).
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.2.3; Kinase, other
Cellular Component: cytosol; lipid raft; membrane
Molecular Function: ADP binding; ATP binding; phosphoglycerate kinase activity
Biological Process: carbohydrate metabolic process; gluconeogenesis; glycolysis
Research Articles on PGK1
Similar Products
Product Notes
The Mouse PGK1 pgk1 (Catalog #AAA9717370) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717370 ELISA Kit recognizes Mouse PGK1. It is sometimes possible for the material contained within the vial of "Phosphoglycerate Kinase 1 (PGK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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