Rabbit anti-Human ZFP57 Polyclonal Antibody | anti-ZFP57 antibody

ZFP57, ID (ZFP57, C6orf40, ZNF698, Zinc finger protein 57 homolog, Zinc finger protein 698) (AP)

Cat. Num. AAA6365161

• Gene Names: ZFP57; TNDM1; ZNF698; C6orf40; bA145L22; bA145L22.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZFP57; Polyclonal Antibody; ID (ZFP57; C6orf40; ZNF698; Zinc finger protein 57 homolog; Zinc finger protein 698) (AP); Zinc finger protein 698; anti-ZFP57 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ZFP57 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ZFP57 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-296 amino acids from the Central region of human ZFP57.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ZFP57 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the ZFP57 antibody detected the ZFP57 protein (arrow).)

Related Product Information for anti-ZFP57 antibody

The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).

Product Categories/Family for anti-ZFP57 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Spengler, S., et al. Eur J Med Genet 52(6):415-416(2009). Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009): Tse, K.P., et al. Am. J. Hum. Genet. 85(2):194-203(2009). Li, X., et al. Dev. Cell 15(4):547-557(2008). Mackay, D.J., et al. Nat. Genet. 40(8):949-951(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 239582722
• NCBI GeneID: 346171
• NCBI Accession #: NP_001103279.2
• NCBI GenBank Nucleotide #: NM_001109809.2
• UniProt Accession #: Q9NU63; Q5SSB1; B0S894; B0V254; B2RXJ7
• Molecular Weight: 51,919 Da
• NCBI Official Full Name: zinc finger protein 57 homolog
• NCBI Official Synonym Full Names: ZFP57 zinc finger protein
• NCBI Official Symbol: ZFP57
• NCBI Official Synonym Symbols: TNDM1; ZNF698; C6orf40; bA145L22; bA145L22.2
• NCBI Protein Information: zinc finger protein 57 homolog; zfp-57; zinc finger protein 698
• UniProt Protein Name: Zinc finger protein 57 homolog
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZFP57
• UniProt Synonym Gene Names: C6orf40; ZNF698; Zfp-57
• UniProt Entry Name: ZFP57_HUMAN

Uniprot Description

ZFP57: Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions. Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Defects in ZFP57 are the cause of transient neonatal diabetes mellitus type 1 (TNDM1). Neonatal diabetes is a form of diabetes mellitus defined by the onset of mild-to- severe hyperglycemia within the first months of life. In about half of the neonates, diabetes is transient and resolves at a median age of 3 months, whereas the rest have a permanent form of diabetes. The major cause of TNDM1 is aberrant expression of imprinted genes at chromosome 6q24, associated in 20% of cases with DNA hypomethylation at the transient neonatal diabetes differentially methylated region (DMR), which lies within the imprinted promoter of the PLAGL1 gene. Over 50% of individuals with transient neonatal diabetes and hypomethylation at 6q24 also show mosaic DNA hypomethylation at other imprinted loci throughout the genome and a range of additional clinical features. Belongs to the krueppel C2H2-type zinc-finger protein family. ZFP57 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: nuclear heterochromatin

Molecular Function: DNA binding; metal ion binding

Biological Process: genetic imprinting; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; DNA methylation during embryonic development; peripheral nervous system development

Disease: Diabetes Mellitus, Transient Neonatal, 1