ADH7 sirna

ADH7 siRNA (Mouse)

Cat. Num. AAA8227488

• Gene Names: Adh7; Adh3; Adh4; Adh-3; Adt-1; Adh-3e; Adh-3t; Adh3-e; Adh3-t; AI325182
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADH7; ADH7 siRNA (Mouse); ADH-3; ADH3; Alcohol dehydrogenase class 4 mu/sigma chain; ADH-C2; Alcohol dehydrogenase 7; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Retinol dehydrogenase; ADH7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ADH7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 374

• Applicable Applications for ADH7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ADH7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADH7 sirna

siRNA to inhibit ADH7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31560625
• NCBI GeneID: 11529
• NCBI Accession #: NP_033756.2
• NCBI GenBank Nucleotide #: NM_009626.4
• UniProt Accession #: Q64437
• Molecular Weight: 39,904 Da
• NCBI Official Full Name: alcohol dehydrogenase class 4 mu/sigma chain
• NCBI Official Synonym Full Names: alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• NCBI Official Symbol: Adh7
• NCBI Official Synonym Symbols: Adh3; Adh4; Adh-3; Adt-1; Adh-3e; Adh-3t; Adh3-e; Adh3-t; AI325182
• NCBI Protein Information: alcohol dehydrogenase class 4 mu/sigma chain
• UniProt Protein Name: Alcohol dehydrogenase class 4 mu/sigma chain
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: Adh7
• UniProt Synonym Gene Names: Adh-3; Adh3
• UniProt Entry Name: ADH7_MOUSE

Uniprot Description

ADH7: Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism. Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.1; Oxidoreductase

Cellular Component: cytoplasm; extracellular region; intracellular

Molecular Function: receptor antagonist activity; protein homodimerization activity; zinc ion binding; aldehyde oxidase activity; metal ion binding; alcohol dehydrogenase activity; oxidoreductase activity; alcohol dehydrogenase activity, zinc-dependent; ethanol binding; NAD binding; retinol binding; retinol dehydrogenase activity

Biological Process: response to ethanol; ethanol catabolic process; response to bacterium; retinol metabolic process; ethanol metabolic process; retinoic acid metabolic process; retinoid metabolic process; ethanol oxidation; fatty acid omega-oxidation