Growth Factor Receptor Bound Protein 7 (Grb7) Recombinant Protein | Grb7 recombinant protein

Recombinant Growth Factor Receptor Bound Protein 7 (Grb7)

Cat. Num. AAA2009313

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Growth Factor Receptor Bound Protein 7 (Grb7); Recombinant Growth Factor Receptor Bound Protein 7 (Grb7); Grb7 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-SIPNP FPELCSPPSQ SPILGGPSSA RGLLPRDASR PHVVKVYSED GACRSVEVAA GATARHVCEM LVQRAHALSD ETWGLVECHP HLALERGLED HESVVEVQAA WPVGGDSRFV FRKNFAKYEL FKSSPHSLFP EKMVSSCLDA HTGISHEDLI QNFLNAGSFP EIQGFLQLRG SGRKLWKRFF CFLRRSGLYY STKGTSKDPR HLQYVADVNE SNVYVVTQGR KLYGMPTDFG FCVKPNKLRN GHKGLRIFCS EDEQSRT
• Sequence Length: 532

• Applicable Applications for Grb7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser66~Thr327 (Accession # Q14451) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 71979667
• NCBI GeneID: 2886
• NCBI Accession #: NP_001025173.1
• NCBI GenBank Nucleotide #: NM_001030002.2
• UniProt Accession #: Q14451; Q53YD3; Q92568; Q96DF9; Q9Y220; B2RAV1; B3KNL0; B3KWP9; B7WP75; J3KQM4
• Molecular Weight: 33.1kDa
• NCBI Official Full Name: growth factor receptor-bound protein 7 isoform a
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 7
• NCBI Official Symbol: GRB7
• NCBI Protein Information: growth factor receptor-bound protein 7; B47; GRB7 adapter protein; epidermal growth factor receptor GRB-7
• UniProt Protein Name: Growth factor receptor-bound protein 7
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB7
• UniProt Entry Name: GRB7_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

GRB7: Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress. Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1. Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Belongs to the GRB7/10/14 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: focal adhesion; cell projection; stress granule; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein kinase binding; phosphoinositide binding

Biological Process: epidermal growth factor receptor signaling pathway; stress granule assembly; positive regulation of signal transduction; negative regulation of translation; blood coagulation; leukocyte migration; positive regulation of cell migration