ARMC10 sirna

ARMC10 siRNA (Human)

Cat. Num. AAA8226379

• Gene Names: ARMC10; SVH; PNAS112; PNAS-112; PSEC0198
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARMC10; ARMC10 siRNA (Human); SVH; Armadillo repeat-containing protein 10; Splicing variant involved in hepatocarcinogenesis protein; ARMC10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARMC10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 225

• Applicable Applications for ARMC10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARMC10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARMC10 sirna

siRNA to inhibit ARMC10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 238776793
• NCBI GeneID: 83787
• NCBI Accession #: NP_001154481.1
• NCBI GenBank Nucleotide #: NM_001161009.2
• UniProt Accession #: Q8N2F6; Q75K91; Q75MG6; Q75ML8; Q8IZC1; Q8IZC2; Q8IZC3; Q9BTM6; A8K703; B4DWJ8; F5GX65
• Molecular Weight: 24,757 Da
• NCBI Official Full Name: armadillo repeat-containing protein 10 isoform b
• NCBI Official Synonym Full Names: armadillo repeat containing 10
• NCBI Official Symbol: ARMC10
• NCBI Official Synonym Symbols: SVH; PNAS112; PNAS-112; PSEC0198
• NCBI Protein Information: armadillo repeat-containing protein 10
• UniProt Protein Name: Armadillo repeat-containing protein 10
• Protein Family: Armadillo repeat-containing protein
• UniProt Gene Name: ARMC10
• UniProt Synonym Gene Names: SVH
• UniProt Entry Name: ARM10_HUMAN

NCBI Description

This gene encodes a protein that contains an armadillo repeat and transmembrane domain. The encoded protein decreases the transcriptional activity of the tumor suppressor protein p53 through direct interaction with the DNA-binding domain of p53, and may play a role in cell growth and survival. Upregulation of this gene may play a role in hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. [provided by RefSeq, Sep 2011]

Uniprot Description

ARMC10: May play a role in cell survival and cell growth. May suppress the transcriptional activity of p53/TP53. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: endoplasmic reticulum membrane; mitochondrion; integral to membrane

Biological Process: regulation of growth