Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DDX56 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Rabbit DDX56 Polyclonal Antibody | anti-DDX56 antibody
DDX56 antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
DDX56 antibody was purified by antigen-affinity chromatography
Synonyms
DDX56; Polyclonal Antibody; DDX56 antibody; Polyclonal DDX56; Anti-DDX56; DDX-56; NOH61; DDX 56; DEAD-box RNA helicase; DEAD box protein 21; DDX21; putative nucleolar RNA helicase; ATP-dependent 61 kDa nucleolar RNA helicase; 61-kd nucleolar helicase; DDX26; anti-DDX56 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
DDX56 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.96 mg/ml (varies by lot)
Sequence Length
547
Applicable Applications for anti-DDX56 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:1000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Biological Significance
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DDX56 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DDX56 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and DDX56 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and DDX56 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded MCF7 xenograft using DDX56 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded MCF7 xenograft using DDX56 antibody at a dilution of 1:500)
Related Product Information for anti-DDX56 antibody
Affinity purified Rabbit polyclonal DDX56 antibody
Product Categories/Family for anti-DDX56 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
62 kDa
NCBI Official Full Name
DDX56
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX56
Protein Family
UniProt Gene Name
DDX56
UniProt Synonym Gene Names
DDX21; NOH61
UniProt Entry Name
DDX56_HUMAN
Uniprot Description
DDX56: May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Protein type: EC 3.6.4.13; Helicase; Nucleolus
Chromosomal Location of Human Ortholog: 7p13
Cellular Component: membrane; nucleolus
Molecular Function: ATP-dependent RNA helicase activity; ATP binding
Biological Process: rRNA processing
Similar Products
Product Notes
The DDX56 ddx56 (Catalog #AAA837302) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DDX56 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:1000 ICC:1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the DDX56 ddx56 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX56, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
