Flow Cytometry (FC/FACS)
(Mouse neutrophils are depleted in vivo by MBS6120614. Method: Mice were injected) i.p. with 250ug of MBS6120614 (B) or with Control mAb (A) in BALB/c mouse 6h prior to Leishmania major infection (3x10e6 parasites injected in the hind footpad). 3 days later, blood (100ul) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).)
anti-Mouse Neutrophils Monoclonal Antibody
Rat Anti-Neutrophils, blocking (Preservative-free)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry
Purity
Purified by Protein G affinity chromatography.
Synonyms
Neutrophils; Monoclonal Antibody; Rat Anti-Neutrophils; blocking (Preservative-free); anti-Neutrophils antibody
Host
Host: Rat
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
Nimp-R14
Specificity
Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 10% glycerol. No preservative added.
Applicable Applications for anti-Neutrophils antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
Flow Cytometry: 1:100 Immunohistochemistry (frozen/paraffin): 1:200 Immunocytochemistry: 1:200 Optimal dilutions to be determined by researcher.
Endotoxin
<0.1EU/ug purified protein (LAL test; Lonza)
Immunogen
Purified mouse BALB/c neutrophils.
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Flow Cytometry (FC/FACS)
(Mouse neutrophils are depleted in vivo by MBS6120614. Method: Mice were injected) i.p. with 250ug of MBS6120614 (B) or with Control mAb (A) in BALB/c mouse 6h prior to Leishmania major infection (3x10e6 parasites injected in the hind footpad). 3 days later, blood (100ul) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).)
Flow Cytometry (FC/FACS)
(Mouse neutrophils are depleted in vivo by MBS6120614. Method: Mice were injected) i.p. with 250ug of MBS6120614 (B) or with Control mAb (A) in BALB/c mouse 6h prior to Leishmania major infection (3x10e6 parasites injected in the hind footpad). 3 days later, blood (100ul) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).)
Related Product Information for anti-Neutrophils antibody
Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.
References
Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies: A. Lopez, et al.; Brit. J. Haematol. 57, 489 (1984)|
Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.; Parasite Immunol. 9, 667 (1987)|
Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997)|?Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999)|?An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000)|?A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000)|?Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003)|?Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003)|?Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004)|?Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.; Invest. Ophthalmol. Vis. Sci. 46, 589 (2005)|?Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008)|?Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009)|?Analysis of innate defences against Plasmodium falciparum in immunodeficient mice: A Ludovic, et al.; Malaria J. 9, 197 (2010)|?Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)
Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.; Parasite Immunol. 9, 667 (1987)|
Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997)|?Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999)|?An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000)|?A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000)|?Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003)|?Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003)|?Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004)|?Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.; Invest. Ophthalmol. Vis. Sci. 46, 589 (2005)|?Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008)|?Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009)|?Analysis of innate defences against Plasmodium falciparum in immunodeficient mice: A Ludovic, et al.; Malaria J. 9, 197 (2010)|?Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)
Similar Products
Product Notes
The Neutrophils (Catalog #AAA6120614) is an Antibody produced from Host: Rat Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Neutrophils, blocking (Preservative-free) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neutrophils can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC). Flow Cytometry: 1:100 Immunohistochemistry (frozen/paraffin): 1:200 Immunocytochemistry: 1:200 Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Neutrophils for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neutrophils, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
