TBATA sirna

TBATA siRNA (Mouse)

Cat. Num. AAA8223402

• Gene Names: Tbata; Titest; Spatial; AI428928; 1700021K02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBATA; TBATA siRNA (Mouse); SPATIAL; Protein TBATA; Protein SPATIAL; Stromal protein associated with thymii and lymph node; Thymus. brain and testes-associated protein; TBATA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TBATA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 197

• Applicable Applications for TBATA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TBATA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBATA sirna

siRNA to inhibit TBATA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 63079693
• NCBI GeneID: 65971
• NCBI Accession #: NP_001017407.1
• NCBI GenBank Nucleotide #: NM_001017407.1
• UniProt Accession #: Q7TSD4; Q6XBG6; Q6XBG7; Q6XBG8; Q7TSD5; Q8BYU0; Q9EQE4; Q9EQE5; B2ZA64
• Molecular Weight: 19,081 Da
• NCBI Official Full Name: protein TBATA isoform 5
• NCBI Official Synonym Full Names: thymus, brain and testes associated
• NCBI Official Symbol: Tbata
• NCBI Official Synonym Symbols: Titest; Spatial; AI428928; 1700021K02Rik
• NCBI Protein Information: protein TBATA
• UniProt Protein Name: Protein TBATA
• Protein Family: Protein
• UniProt Gene Name: Tbata
• UniProt Synonym Gene Names: Spatial
• UniProt Entry Name: TBATA_MOUSE

NCBI Description

This gene encodes a putative transcription factor that is highly expressed in thymic cortical stromal cells, and may be involved in T-cell development. Its expression is developmentally regulated in the testis, where it is restricted to the haploid round spermatids during spermatogenesis, and thus this gene may also have a role in the control of male germ cell development. Alternative splicing of this gene results in two sets of transcript variants: the variants containing 5 additional exons at the 3' end encode long isoforms that are highly expressed in the testis, while the variants lacking the 3' end exons encode short isoforms that are highly expressed in the thymus. Most of the transcripts encoding the short isoforms have been shown to initiate translation from non-AUG (CUG) start sites. [provided by RefSeq, Jul 2008]

Uniprot Description

SPATIAL: May play a role in spermatid differentiation. Modulates thymic stromal cell proliferation and thymus function. Belongs to the TBATA family. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding

Biological Process: spermatid differentiation; multicellular organismal development; spermatogenesis; cell differentiation