HTR2C sirna

HTR2C siRNA (Rat)

Cat. Num. AAA8223222

• Gene Names: Htr2c; 5-HT2C; 5HT-1C; 5-HTR2C
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HTR2C; HTR2C siRNA (Rat); 5HT1C; HTR1C; 5-hydroxytryptamine receptor 2C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-hydroxytryptamine receptor 1C; 5-HT-1C; 5-HT1C; Serotonin receptor 2C; HTR2C sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HTR2C siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 460

• Applicable Applications for HTR2C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HTR2C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HTR2C sirna

siRNA to inhibit HTR2C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17298688
• NCBI GeneID: 25187
• NCBI Accession #: NP_036897.2
• NCBI GenBank Nucleotide #: NM_012765.3
• UniProt Accession #: P08909
• Molecular Weight: 51,917 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 2C
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
• NCBI Official Symbol: Htr2c
• NCBI Official Synonym Symbols: 5-HT2C; 5HT-1C; 5-HTR2C
• NCBI Protein Information: 5-hydroxytryptamine receptor 2C
• UniProt Protein Name: 5-hydroxytryptamine receptor 2C
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: Htr2c
• UniProt Synonym Gene Names: 5ht1c; Htr1c; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C
• UniProt Entry Name: 5HT2C_RAT

NCBI Description

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations. [provided by RefSeq, Jul 2008]

Uniprot Description

5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: cell surface; cell; integral to plasma membrane; plasma membrane; cytosol; external side of plasma membrane

Molecular Function: serotonin binding; protein binding; serotonin receptor activity; drug binding; 5-HT2 receptor activity

Biological Process: response to drug; cGMP biosynthetic process; organ regeneration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inositol phosphate-mediated signaling; behavioral fear response; G-protein signaling, phospholipase D activating pathway; regulation of corticotropin-releasing hormone secretion; serotonin receptor, phospholipase C activating pathway; behavioral response to nicotine; locomotory behavior; positive regulation of phosphatidylinositol biosynthetic process; negative regulation of dopamine metabolic process; cellular calcium ion homeostasis; regulation of neurological process; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of locomotion; release of sequestered calcium ion into cytosol; regulation of appetite; positive regulation of vasoconstriction; positive regulation of fat cell differentiation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; regulation of sensory perception of pain; serotonin receptor signaling pathway; positive regulation of acetylcholine secretion; positive regulation of gamma-aminobutyric acid secretion