Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit | HTR2C elisa kit

Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit

Cat. Num. AAA450248

• Gene Names: Htr2c; 5-HT2C; 5HT-1C; 5-HTR2C
• Reactivity: Rat
• Synonyms: 5-Hydroxytryptamine Receptor 2C (HTR2C); Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit; 5-HT2C; HTR1C; Serotonin Receptor 2C; 5-hydroxytryptamine receptor 1C; HTR2C elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of HTR2C. No significant cross-reactivity or interference between HTR2C and analogues was observed.
• Sequence Length: 460

• Samples: Rat tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.083ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HTR2C were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HTR2C were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HTR2C elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HTR2C in rat tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTR2C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HTR2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTR2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HTR2C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17298688
• NCBI GeneID: 25187
• NCBI Accession #: NP_036897.2
• NCBI GenBank Nucleotide #: NM_012765.3
• UniProt Accession #: P08909
• Molecular Weight: 51,917 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 2C
• NCBI Official Synonym Full Names: 5-hydroxytryptamine receptor 2C
• NCBI Official Symbol: Htr2c
• NCBI Official Synonym Symbols: 5-HT2C; 5HT-1C; 5-HTR2C
• NCBI Protein Information: 5-hydroxytryptamine receptor 2C
• UniProt Protein Name: 5-hydroxytryptamine receptor 2C
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: Htr2c
• UniProt Synonym Gene Names: 5ht1c; Htr1c; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C

NCBI Description

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations. [provided by RefSeq, Jul 2008]

Uniprot Description

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and feeding behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.