HOPX sirna

HOPX siRNA (Human)

Cat. Num. AAA8222563

• Gene Names: HOPX; HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HOPX; HOPX siRNA (Human); HOD; HOP; LAGY; NECC1; OB1; Homeodomain-only protein; Lung cancer-associated Y protein; Not expressed in choriocarcinoma protein 1; Odd homeobox protein 1; HOPX sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HOPX siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 73

• Applicable Applications for HOPX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HOPX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HOPX sirna

siRNA to inhibit HOPX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224451023
• NCBI GeneID: 84525
• NCBI Accession #: NP_001138931.1
• NCBI GenBank Nucleotide #: NM_001145459.1
• UniProt Accession #: Q9BPY8; Q8N0V6; Q96CI1; A8K0Z2; E9PB55; G3V294
• Molecular Weight: 12,625 Da
• NCBI Official Full Name: homeodomain-only protein isoform b
• NCBI Official Synonym Full Names: HOP homeobox
• NCBI Official Symbol: HOPX
• NCBI Official Synonym Symbols: HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31
• NCBI Protein Information: homeodomain-only protein
• UniProt Protein Name: Homeodomain-only protein
• Protein Family: Homeodomain-only protein
• UniProt Gene Name: HOPX
• UniProt Synonym Gene Names: HOD; HOP; LAGY; NECC1; OB1
• UniProt Entry Name: HOP_HUMAN

NCBI Description

The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

HOPX: Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy. May act as a tumor suppressor. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: negative regulation of cell differentiation; positive regulation of skeletal muscle regeneration; transcription, DNA-dependent; regulation of protein binding; heart development; histone deacetylation; positive regulation of striated muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; alveolus development; regulation of heart contraction; trophectodermal cell differentiation