PTPN13 sirna

PTPN13 siRNA (Human)

Cat. Num. AAA8220143

• Gene Names: PTPN13; PNP1; FAP-1; PTP1E; PTPL1; PTPLE; PTP-BL; hPTP1E; PTP-BAS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPN13; PTPN13 siRNA (Human); PNP1; PTP1E; PTPL1; Tyrosine-protein phosphatase non-receptor type 13; Fas-associated protein-tyrosine phosphatase 1; FAP-1; PTP-BAS; Protein-tyrosine phosphatase 1E; PTP-E1; hPTPE1; Protein-tyrosine phosphatase PTPL1; PTPN13 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PTPN13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2466

• Applicable Applications for PTPN13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PTPN13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPN13 sirna

siRNA to inhibit PTPN13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5453992
• NCBI GeneID: 5783
• NCBI Accession #: NP_006255.1
• NCBI GenBank Nucleotide #: NM_006264.2
• UniProt Accession #: Q12923; Q15159; Q15263; Q15264; Q15265; Q15674; Q16826; Q8IWH7; Q9NYN9; Q9UDA8; B2RTR0
• Molecular Weight: 277,508 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 13 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase)
• NCBI Official Symbol: PTPN13
• NCBI Official Synonym Symbols: PNP1; FAP-1; PTP1E; PTPL1; PTPLE; PTP-BL; hPTP1E; PTP-BAS
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 13
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 13
• Protein Family: PTPN13-like protein
• UniProt Gene Name: PTPN13
• UniProt Synonym Gene Names: PNP1; PTP1E; PTPL1; FAP-1; PTP-E1; hPTPE1
• UniProt Entry Name: PTN13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large intracellular protein. It has a catalytic PTP domain at its C-terminus and two major structural domains: a region with five PDZ domains and a FERM domain that binds to plasma membrane and cytoskeletal elements. This PTP was found to interact with, and dephosphorylate, Fas receptor and IkappaBalpha through the PDZ domains. This suggests it has a role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathways. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Oct 2008]

Uniprot Description

PTPN13: Tyrosine phosphatase which regulates negatively FAS- induced apoptosis and NGFR-mediated pro-apoptotic signaling. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: neuron projection; cytoskeleton; lamellipodium; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: regulation of phosphoinositide 3-kinase cascade; protein amino acid dephosphorylation