PPARD sirna

PPARD siRNA (Mouse)

Cat. Num. AAA8217857

• Gene Names: Ppard; NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPARD; PPARD siRNA (Mouse); NR1C2; PPARB; Peroxisome proliferator-activated receptor delta; PPAR-delta; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta; PPARD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PPARD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 440

• Applicable Applications for PPARD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PPARD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPARD sirna

siRNA to inhibit PPARD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33859590
• NCBI GeneID: 19015
• NCBI Accession #: NP_035275.1
• NCBI GenBank Nucleotide #: NM_011145.3
• UniProt Accession #: P35396; P37239
• Molecular Weight: 49,715 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor delta
• NCBI Official Synonym Full Names: peroxisome proliferator activator receptor delta
• NCBI Official Symbol: Ppard
• NCBI Official Synonym Symbols: NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta
• NCBI Protein Information: peroxisome proliferator-activated receptor delta
• UniProt Protein Name: Peroxisome proliferator-activated receptor delta
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: Ppard
• UniProt Synonym Gene Names: Nr1c2; Pparb; PPAR-delta; NUC1; PPAR-beta
• UniProt Entry Name: PPARD_MOUSE

Uniprot Description

PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Cellular Component: nucleus

Molecular Function: retinoid X receptor binding; NF-kappaB binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; prostacyclin receptor activity; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; enzyme activator activity; transcription coactivator activity; steroid hormone receptor activity; drug binding; lipid binding; transcription factor activity; fatty acid binding

Biological Process: positive regulation of catalytic activity; transcription from RNA polymerase II promoter; proteoglycan metabolic process; epidermis development; wound healing; negative regulation of smooth muscle cell proliferation; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; vitamin A metabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; axon ensheathment; cell differentiation; placenta development; negative regulation of epithelial cell proliferation; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of insulin secretion; keratinocyte proliferation; cell-substrate adhesion; keratinocyte migration; regulation of cell proliferation; phospholipid biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; regulation of transcription from RNA polymerase II promoter; G-protein coupled receptor protein signaling pathway; cell proliferation; negative regulation of inflammatory response; mRNA transcription; steroid hormone mediated signaling; fatty acid oxidation; negative regulation of cell growth; lipid metabolic process; anagen; regulation of insulin secretion; cellular process; embryo implantation; negative regulation of apoptosis