Flow Cytometry (FC/FACS)
(Flow cytometry analysis (intracellular staining) of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.)
Mouse anti-Human HLA-G Monoclonal Antibody | anti-HLA-G antibody
Anti-HLA-G Biotin
Gene Names
HLA-G; MHC-G
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, ELISA
Purity
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Synonyms
HLA-G; Monoclonal Antibody; Anti-HLA-G Biotin; major histocompatibility complex; class I; G; anti-HLA-G antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5A6G7
Specificity
The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Purity/Purification
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
338
Applicable Applications for anti-HLA-G antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
FC/FACS: QC tested
Immunogen
C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
Conjugation
Biotin
Preparation and Storage
Store at 2-8 degree C.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (intracellular staining) of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (intracellular staining) of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
HLA class I histocompatibility antigen, alpha chain G
NCBI Official Synonym Full Names
major histocompatibility complex, class I, G
NCBI Official Symbol
HLA-G
NCBI Official Synonym Symbols
MHC-G
NCBI Protein Information
HLA class I histocompatibility antigen, alpha chain G
UniProt Protein Name
HLA class I histocompatibility antigen, alpha chain G
Protein Family
UniProt Gene Name
HLA-G
UniProt Synonym Gene Names
HLA-6.0; HLAG
UniProt Entry Name
HLAG_HUMAN
NCBI Description
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Research Articles on HLA-G
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Product Notes
The HLA-G hla-g (Catalog #AAA1800250) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA-G Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), ELISA (EIA). FC/FACS: QC tested. Researchers should empirically determine the suitability of the HLA-G hla-g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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