Peroxisome Proliferator Activated Receptor Delta (PPARd) Recombinant Protein | PPARd recombinant protein

Recombinant Peroxisome Proliferator Activated Receptor Delta (PPARd)

Cat. Num. AAA2011232

• Gene Names: Ppard; NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peroxisome Proliferator Activated Receptor Delta (PPARd); Recombinant Peroxisome Proliferator Activated Receptor Delta (PPARd); PPARd recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS- T EFAKNIPNFS SLFLNDQVTL LKYGVHEAIF AMLASIVNKD GLLVANGSGF VTHEFLRSLR KPFSDIIEPK FEFAVKFNAL ELDDSDLALF IAAIILCGDR PGLMNVPQVE AIQDTILRAL EFHLQVNHPD SQYLFPKLLQ KMADLRQLVT EHAQMMQWLK KTESET
• Sequence Length: 440

• Applicable Applications for PPARd recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Thr260~Thr426 (Accession # P35396) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 33859590
• NCBI GeneID: 19015
• NCBI Accession #: NP_035275.1
• NCBI GenBank Nucleotide #: NM_011145.3
• UniProt Accession #: P35396; P37239
• Molecular Weight: 20.2kDa
• NCBI Official Full Name: peroxisome proliferator-activated receptor delta
• NCBI Official Synonym Full Names: peroxisome proliferator activator receptor delta
• NCBI Official Symbol: Ppard
• NCBI Official Synonym Symbols: NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta
• NCBI Protein Information: peroxisome proliferator-activated receptor delta; PPARdelta/beta; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta
• UniProt Protein Name: Peroxisome proliferator-activated receptor delta
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: Ppard
• UniProt Synonym Gene Names: Nr1c2; Pparb; PPAR-delta; NUC1; PPAR-beta
• UniProt Entry Name: PPARD_MOUSE

Uniprot Description

PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Cellular Component: nucleus

Molecular Function: ligand-dependent nuclear receptor activity; zinc ion binding; prostacyclin receptor activity; metal ion binding; transcription coactivator activity; enzyme activator activity; drug binding; retinoid X receptor binding; NF-kappaB binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; steroid hormone receptor activity; fatty acid binding; lipid binding; transcription factor activity

Biological Process: positive regulation of catalytic activity; transcription from RNA polymerase II promoter; proteoglycan metabolic process; epidermis development; wound healing; negative regulation of smooth muscle cell proliferation; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; vitamin A metabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; axon ensheathment; cell differentiation; placenta development; negative regulation of epithelial cell proliferation; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of insulin secretion; keratinocyte proliferation; keratinocyte migration; cell-substrate adhesion; regulation of cell proliferation; phospholipid biosynthetic process; regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; cell proliferation; negative regulation of inflammatory response; mRNA transcription; steroid hormone mediated signaling; fatty acid oxidation; negative regulation of cell growth; lipid metabolic process; anagen; regulation of insulin secretion; cellular process; embryo implantation; negative regulation of apoptosis