AIF1 sirna

AIF1 siRNA (Human)

Cat. Num. AAA8212438

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AIF1; AIF1 siRNA (Human); G1; IBA1; Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AIF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 147

• Applicable Applications for AIF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AIF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AIF1 sirna

siRNA to inhibit AIF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14574568
• NCBI GeneID: 199
• NCBI Accession #: NP_001614.3
• NCBI GenBank Nucleotide #: NM_001623.3
• UniProt Accession #: P55008; O43904; Q9UIV4; Q9UKS9; A8K406
• Molecular Weight: 14,617 Da
• NCBI Official Full Name: allograft inflammatory factor 1 isoform 3
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Synonym Gene Names: G1; IBA1; AIF-1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; perikaryon; actin filament; cytosol; nucleus; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; inflammatory response; response to electrical stimulus; negative regulation of apoptosis