Rabbit MMP16 Polyclonal Antibody | anti-MMP16 antibody

MMP16, NT (Matrix Metalloproteinase-16, MMP-16, Membrane-type Matrix Metalloproteinase 3, MT-MMP 3, MTMMP3, Membrane-type-3 Matrix Metalloproteinase, MT3-MMP, MT3MMP, MMP-X2, MMPX2)

Cat. Num. AAA624817

• Gene Names: MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP; DKFZp761D112
• Reactivity: Hamster, Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MMP16; Polyclonal Antibody; NT (Matrix Metalloproteinase-16; MMP-16; Membrane-type Matrix Metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 Matrix Metalloproteinase; MT3-MMP; MT3MMP; MMP-X2; MMPX2); Anti -MMP16; anti-MMP16 antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP16. Species Crossreactivity: hamster.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MMP16 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human MMP16 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(MMP16 Antibody (E169) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MMP16 antibody detected the MMP16 protein (arrow).)

Related Product Information for anti-MMP16 antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene for MMP16 produces two transcripts, which encode a membrane-bound form and a soluble form of the protein. Both forms of the protein activate MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.

Product Categories/Family for anti-MMP16 antibody

Antibodies; Abs to Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 13027802
• NCBI GeneID: 4325
• NCBI Accession #: NP_005932.2
• NCBI GenBank Nucleotide #: NM_005941.4
• UniProt Accession #: P51512; Q14824; Q52H48; B2RAN7
• Molecular Weight: 69,521 Da
• NCBI Official Full Name: matrix metalloproteinase-16
• NCBI Official Synonym Full Names: matrix metallopeptidase 16 (membrane-inserted)
• NCBI Official Symbol: MMP16
• NCBI Official Synonym Symbols: MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP; DKFZp761D112
• NCBI Protein Information: matrix metalloproteinase-16; MMP-16; MT3MMP; MTMMP3; MT-MMP 3; OTTHUMP00000224791; Putative transmembrane protein C8orf57; membrane-type matrix metalloproteinase 3; membrane-type-3 matrix metalloproteinase
• UniProt Protein Name: Matrix metalloproteinase-16
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP16
• UniProt Synonym Gene Names: MMPX2
• UniProt Entry Name: MMP16_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq]

Uniprot Description

MMP16: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: proteinaceous extracellular matrix; cell surface; Golgi lumen; integral to plasma membrane; plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; embryonic cranial skeleton morphogenesis; proteolysis; endochondral ossification