Western Blot (WB)
(Western blot analysis of Endothelin A Receptor expression in NCIH460 (A), HEK293 (B) whole cell lysates.)
Rabbit anti-Human Endothelin A Receptor Polyclonal Antibody | anti-EDNRA antibody
Anti-Endothelin A Receptor Antibody
Western Blot (WB)
(Western blot analysis of Endothelin A Receptor expression in NCIH460 (A), HEK293 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Endothelin A Receptor expression in NCIH460 (A), HEK293 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Endothelin A Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Endothelin A Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Uniprot Description
EDNRA: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q31.22
Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; lipid raft
Molecular Function: endothelin receptor activity; protein binding; phosphoinositide phospholipase C activity
Biological Process: positive regulation of odontogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); neural crest cell development; metabolic process; heart development; smooth muscle cell proliferation; response to morphine; response to lipopolysaccharide; glucose transport; signal transduction; sensory perception of pain; enteric nervous system development; Rho protein signal transduction; regulation of epithelial cell proliferation; histamine secretion; negative regulation of cAMP biosynthetic process; elevation of cytosolic calcium ion concentration; smooth muscle contraction; fibroblast proliferation; regulation of blood pressure; positive regulation of cell proliferation; protein kinase C activation; artery smooth muscle contraction; aging; penile erection; vasoconstriction; in utero embryonic development; adenylate cyclase activation; glomerular filtration; respiratory gaseous exchange; patterning of blood vessels; cell proliferation; G-protein coupled receptor protein signaling pathway; phospholipase C activation; maternal process involved in parturition; response to hypoxia; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of inflammatory response; negative regulation of apoptosis
Disease: Mandibulofacial Dysostosis With Alopecia; Migraine With Or Without Aura, Susceptibility To, 1
Research Articles on EDNRA
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Product Notes
The EDNRA ednra (Catalog #AAA821157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Endothelin A Receptor Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Endothelin A Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the EDNRA ednra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endothelin A Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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