HINFP sirna

HINFP siRNA (Mouse)

Cat. Num. AAA8211450

• Gene Names: Hinfp; Mizf; HiNF-P; AA589481
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HINFP; HINFP siRNA (Mouse); MIZF; Histone H4 transcription factor; Histone nuclear factor P; HiNF-P; MBD2-interacting zinc finger protein; Methyl-CpG-binding protein 2-interacting zinc finger protein; HINFP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HINFP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 503

• Applicable Applications for HINFP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HINFP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HINFP sirna

siRNA to inhibit HINFP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 281306720
• NCBI GeneID: 102423
• NCBI Accession #: NP_751894.2
• NCBI GenBank Nucleotide #: NM_172162.3
• UniProt Accession #: Q8K1K9; Q8BWY0; E9QPQ4
• Molecular Weight: 58,076 Da
• NCBI Official Full Name: histone H4 transcription factor
• NCBI Official Synonym Full Names: histone H4 transcription factor
• NCBI Official Symbol: Hinfp
• NCBI Official Synonym Symbols: Mizf; HiNF-P; AA589481
• NCBI Protein Information: histone H4 transcription factor
• UniProt Protein Name: Histone H4 transcription factor
• Protein Family: Histone H4 transcription factor
• UniProt Gene Name: Hinfp
• UniProt Synonym Gene Names: Mizf; HiNF-P
• UniProt Entry Name: HINFP_MOUSE

Uniprot Description

MIZF: a transcriptional regulator of cell cycle-controlled histone H4 genes at the onset of S phase. Also activates transcription of the ATM and PRKDC genes. A histone H4-specific transcriptional regulator that associates with the cell cycle control element in the conserved proximal promoter regions of H4 genes. Interacts with NPAT to coactivate H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. HiNF-P/NPAT colocalize to Cajal bodies in a cell cycle-specific manner. Disruption of the HiNF-P/NPAT interaction inhibits histone H4 gene expression at G1/S. Binds methyl-CpG-binding protein (MeCP2) and a histone deacetylase complex.

Protein type: Histone-binding; C2H2-type zinc finger protein; Cell cycle regulation

Cellular Component: nucleoplasm; Cajal body; nucleus

Molecular Function: enzyme binding; DNA binding; nucleic acid binding; histone binding; metal ion binding; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; DNA repair; myoblast differentiation; G1/S-specific transcription in mitotic cell cycle; establishment of protein localization; regulation of transcription, DNA-dependent; regulation of gene expression; DNA damage checkpoint; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle