ENG sirna

ENG siRNA (Mouse)

Cat. Num. AAA8211199

• Gene Names: Eng; Endo; CD105; AI528660; AI662476; S-endoglin
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENG; ENG siRNA (Mouse); EDG; Endoglin; Cell surface MJ7/18 antigen; CD antigen CD105; ENG sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ENG siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 653

• Applicable Applications for ENG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ENG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENG sirna

siRNA to inhibit ENG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226437647
• NCBI GeneID: 13805
• NCBI Accession #: NP_031958.2
• NCBI GenBank Nucleotide #: NM_007932.2
• UniProt Accession #: Q63961; Q61520; Q8K100
• Molecular Weight: 70,021 Da
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: Eng
• NCBI Official Synonym Symbols: Endo; CD105; AI528660; AI662476; S-endoglin
• NCBI Protein Information: endoglin
• UniProt Protein Name: Endoglin
• Protein Family: Endoglin
• UniProt Gene Name: Eng
• UniProt Synonym Gene Names: Edg
• UniProt Entry Name: EGLN_MOUSE

Uniprot Description

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Cellular Component: nucleoplasm; extracellular space; cell surface; focal adhesion; membrane; cytoplasm; integral to membrane; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; protein homodimerization activity; transforming growth factor beta binding; galactose binding; punt binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: wound healing; heart development; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; smooth muscle development; extracellular matrix disassembly; regulation of transforming growth factor beta receptor signaling pathway; venous blood vessel morphogenesis; central nervous system vasculogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; negative regulation of protein amino acid autophosphorylation; angiogenesis; heart looping; cell adhesion; vasculogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; cell migration; negative regulation of nitric-oxide synthase activity; patterning of blood vessels; positive regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation