Testing Data
Mouse anti-Chicken CD8 beta Monoclonal Antibody | anti-LOC100136222 antibody
Mouse Anti-Chicken CD8b-FITC
Reactivity
Chicken
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD8 beta; Monoclonal Antibody; Mouse Anti-Chicken CD8b-FITC; Mouse Anti-Chicken CD8b - FITC; Mouse Anti-Chicken CD8 beta; anti-LOC100136222 antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
EP42
Specificity
beta chain (Mr 34 kDa) of chicken CD8
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1,2 MAb EP42 recognizes the CD8 beta chain.1
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1,2 MAb EP42 recognizes the CD8 beta chain.1
Form/Format
Fluorescein Conjugate
Sequence Length
213
Applicable Applications for anti-LOC100136222 antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Testing Data
Testing Data
References
1. Tregaskes, C.A., F. Kong, E. Paramithiotis, C.-L. Chen, M.J.H. Ratcliffe, T. F. Davison, and J.R. Young. 1995. J. Immunol. 154:4485.
2. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
3. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990.. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du
CNRS, pp. 239-249.
4. Ratcliffe. 1996. Personal communication.
2. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
3. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990.. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du
CNRS, pp. 239-249.
4. Ratcliffe. 1996. Personal communication.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD8 beta
NCBI Official Symbol
LOC100136222
NCBI Protein Information
CD8 beta
Similar Products
Product Notes
The LOC100136222 (Catalog #AAA673903) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken CD8b-FITC reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 beta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the LOC100136222 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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