Human WNT Inhibitory Factor 1 (WIF1) ELISA Kit | WIF1 elisa kit

Human WNT Inhibitory Factor 1 (WIF1) ELISA Kit

Cat. Num. AAA2023495

• Gene Names: Wif1; WIF-1; AW107799
• Reactivity: Human
• Synonyms: WNT Inhibitory Factor 1 (WIF1); Human WNT Inhibitory Factor 1 (WIF1) ELISA Kit; WIF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT Inhibitory Factor 1 (WIF1).; No significant cross-reactivity or interference between WNT Inhibitory Factor 1 (WIF1) and analogues was observed.
• Sequence Length: 379

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 5.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT Inhibitory Factor 1 (WIF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT Inhibitory Factor 1 (WIF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WIF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for WIF1 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 6755999
• NCBI GeneID: 24117
• NCBI Accession #: NP_036045.1
• NCBI GenBank Nucleotide #: NM_011915.2
• UniProt Accession #: Q9WUA1
• Molecular Weight: 41,590 Da
• NCBI Official Full Name: wnt inhibitory factor 1
• NCBI Official Synonym Full Names: Wnt inhibitory factor 1
• NCBI Official Symbol: Wif1
• NCBI Official Synonym Symbols: WIF-1; AW107799
• NCBI Protein Information: wnt inhibitory factor 1
• UniProt Protein Name: Wnt inhibitory factor 1
• Protein Family: Wnt inhibitory factor
• UniProt Gene Name: Wif1
• UniProt Synonym Gene Names: WIF-1
• UniProt Entry Name: WIF1_MOUSE

Uniprot Description

WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: extracellular region

Molecular Function: Wnt-protein binding

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development; positive regulation of fat cell differentiation