LDLR sirna

LDLR siRNA (Rat)

Cat. Num. AAA8210731

• Gene Names: Ldlr; LDLRA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LDLR; LDLR siRNA (Rat); Low-density lipoprotein receptor; LDL receptor; LDLR sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: LDLR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 879

• Applicable Applications for LDLR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat LDLR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LDLR sirna

siRNA to inhibit LDLR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28461161
• NCBI GeneID: 300438
• NCBI Accession #: NP_786938.1
• NCBI GenBank Nucleotide #: NM_175762.2
• UniProt Accession #: P35952
• Molecular Weight: 96,622 Da
• NCBI Official Full Name: low-density lipoprotein receptor
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: Ldlr
• NCBI Official Synonym Symbols: LDLRA
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: LDLR chaperone
• UniProt Gene Name: Ldlr
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_RAT

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Cellular Component: Golgi apparatus; cell surface; lysosome; recycling endosome membrane; early endosome; integral to membrane; coated pit; caveola; membrane; late endosome; plasma membrane; endosome; receptor complex; external side of plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; calcium ion binding; glycoprotein binding

Biological Process: receptor-mediated endocytosis; lipoprotein catabolic process; cholesterol metabolic process; response to glucagon stimulus; cholesterol transport; cholesterol absorption; response to hormone stimulus; endocytosis; lipoprotein metabolic process; lipid transport; response to estradiol stimulus; cholesterol homeostasis; response to estrogen stimulus; response to hypoxia; phospholipid transport; lipid metabolic process; aging