Parkinson Disease Protein 7 (PARK7) Recombinant Protein | PARK7 recombinant protein

Recombinant Parkinson Disease Protein 7 (PARK7)

Cat. Num. AAA2010742

• Gene Names: Park7; Dj1; CAP1; DJ-1; SP22
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >97%
• Synonyms: Parkinson Disease Protein 7 (PARK7); Recombinant Parkinson Disease Protein 7 (PARK7); PARK7 recombinant protein

• Host: E Coli
• Purity/Purification: >97%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGSEF-MASKRALVIL AKGAEEMETV IPVDIMRRAG IKVTVAGLAG KDPVQCSRDV VICPDTSLEE AKTQGPYDVV VLPGGNLGAQ NLSESALVKE ILKEQENRKG LIAAICAGPT ALLAHEVGFG CKVTSHPLAK DKMMNGSHYS YSESRVEKDG LILTSRGPGT SFEFALAIVE ALSGKDMANQ VKAPLVLKD
• Sequence Length: 189

• Applicable Applications for PARK7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Asp189 (Accession # O88767) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 471013391
• NCBI GeneID: 117287
• NCBI Accession #: NP_001264179.1
• NCBI GenBank Nucleotide #: NM_001277250.1
• UniProt Accession #: O88767
• Molecular Weight: 21.4kDa
• NCBI Official Full Name: protein DJ-1 isoform 2
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: Park7
• NCBI Official Synonym Symbols: Dj1; CAP1; DJ-1; SP22
• NCBI Protein Information: protein DJ-1; fertility protein SP22; contraception-associated protein 1; parkinson disease protein 7 homolog; Parkinson disease (autosomal recessive, early onset) 7
• UniProt Protein Name: Protein DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: Park7
• UniProt Synonym Gene Names: Cap1; Protein CAP1
• UniProt Entry Name: PARK7_RAT

NCBI Description

displays a specific localization pattern to the anterior-ventral surface of the equatorial segment; may play a role in sperm-egg interactions and fertilization [RGD, Feb 2006]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Tumor suppressor; Transcription regulation; Nuclear receptor co-regulator; EC 3.4.-.-

Cellular Component: PML body; mitochondrion; axon; endoplasmic reticulum; cytoplasm; plasma membrane; chromatin; mitochondrial respiratory chain complex I; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; mercury ion binding; transcription coactivator activity; transcription factor binding; mRNA binding; peptidase activity; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding

Biological Process: synaptic transmission, dopaminergic; negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; response to drug; mitochondrion organization and biogenesis; protein stabilization; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; positive regulation of peptidyl-serine phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein sumoylation; methylglyoxal catabolic process to D-lactate; membrane depolarization; fertilization; response to hydrogen peroxide; detoxification of mercury ion; regulation of inflammatory response; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; autophagy; lactate biosynthetic process; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; response to oxidative stress; negative regulation of apoptosis