Rat Low-density lipoprotein receptor ELISA Kit | Ldlr elisa kit

Rat Low-density lipoprotein receptor ELISA Kit

Cat. Num. AAA2880479

• Gene Names: Ldlr; LDLRA
• Reactivity: Rat
• Synonyms: Low-density lipoprotein receptor; Rat Low-density lipoprotein receptor ELISA Kit; LDL receptor; Ldlr; Ldlr elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat LDL receptor
• Sequence Length: 879

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 1.56 - 100 ng/mL
• Intra-assay Precision: Intra-Assay CV: <4.1%
• Inter-assay Precision: Inter-Assay CV: <6.6%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ldlr elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 28461161
• NCBI GeneID: 300438
• NCBI Accession #: NP_786938.1
• NCBI GenBank Nucleotide #: NM_175762.2
• UniProt Accession #: P35952
• Molecular Weight: 96,622 Da
• NCBI Official Full Name: low-density lipoprotein receptor
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: Ldlr
• NCBI Official Synonym Symbols: LDLRA
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: Ldlr
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_RAT

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell surface; Membrane protein, integral

Cellular Component: apical part of cell; basolateral plasma membrane; caveola; cell surface; coated pit; early endosome; endosome; external side of plasma membrane; Golgi apparatus; integral to membrane; late endosome; lysosome; membrane; plasma membrane; receptor complex; recycling endosome membrane

Molecular Function: calcium ion binding; glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; very-low-density lipoprotein receptor activity

Biological Process: aging; cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; lipid metabolic process; lipid transport; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; receptor-mediated endocytosis; response to estradiol stimulus; response to estrogen stimulus; response to glucagon stimulus; response to hormone stimulus; response to hypoxia