TAF4B sirna

TAF4B siRNA (Human)

Cat. Num. AAA8209153

• Gene Names: TAF4B; SPGF13; TAF2C2; TAFII105
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAF4B; TAF4B siRNA (Human); TAF2C2; TAFII105; Transcription initiation factor TFIID subunit 4B; Transcription initiation factor TFIID 105 kDa subunit; TAF(II)105; TAFII-105; TAF4B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TAF4B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 867

• Applicable Applications for TAF4B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TAF4B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAF4B sirna

siRNA to inhibit TAF4B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 656985069
• NCBI GeneID: 6875
• NCBI Accession #: NP_001280654.1
• NCBI GenBank Nucleotide #: NM_001293725.1
• UniProt Accession #: Q92750; Q29YA4; Q29YA5
• Molecular Weight: 63,364 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 4B isoform 1
• NCBI Official Synonym Full Names: TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa
• NCBI Official Symbol: TAF4B
• NCBI Official Synonym Symbols: SPGF13; TAF2C2; TAFII105
• NCBI Protein Information: transcription initiation factor TFIID subunit 4B
• UniProt Protein Name: Transcription initiation factor TFIID subunit 4B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF4B
• UniProt Synonym Gene Names: TAF2C2; TAFII105; TAF(II)105; TAFII-105; TAFII105
• UniProt Entry Name: TAF4B_HUMAN

NCBI Description

TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

TAF4B: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Belongs to the TAF4 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm; nucleolus

Molecular Function: NF-kappaB binding; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: oogenesis; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; spermatogenesis; gene expression

Disease: Spermatogenic Failure 13