SNX9 sirna

SNX9 siRNA (Human)

Cat. Num. AAA8206258

• Gene Names: SNX9; SDP1; WISP; SH3PX1; SH3PXD3A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNX9; SNX9 siRNA (Human); SH3PX1; SH3PXD3A; Sorting nexin-9; SH3 and PX domain-containing protein 1; Protein SDP1; SH3 and PX domain-containing protein 3A; SNX9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SNX9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 595

• Applicable Applications for SNX9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SNX9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNX9 sirna

siRNA to inhibit SNX9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7706706
• NCBI GeneID: 51429
• NCBI Accession #: NP_057308.1
• NCBI GenBank Nucleotide #: NM_016224.4
• UniProt Accession #: Q9Y5X1; Q9BSI7; Q9BVM1; Q9UJH6; Q9UP20
• Molecular Weight: 66,592 Da
• NCBI Official Full Name: sorting nexin-9
• NCBI Official Synonym Full Names: sorting nexin 9
• NCBI Official Symbol: SNX9
• NCBI Official Synonym Symbols: SDP1; WISP; SH3PX1; SH3PXD3A
• NCBI Protein Information: sorting nexin-9
• UniProt Protein Name: Sorting nexin-9
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX9
• UniProt Synonym Gene Names: SH3PX1; SH3PXD3A; Protein SDP1
• UniProt Entry Name: SNX9_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phosphoinositide binding domain, and are involved in intracellular trafficking. The encoded protein does not contain a coiled coil region, like some family members, but does contain a SRC homology domain near its N-terminus. The encoded protein is reported to have a variety of interaction partners, including of adaptor protein 2 , dynamin, tyrosine kinase non-receptor 2, Wiskott-Aldrich syndrome-like, and ARP3 actin-related protein 3. The encoded protein is implicated in several stages of intracellular trafficking, including endocytosis, macropinocytosis, and F-actin nucleation. [provided by RefSeq, Jul 2013]

Uniprot Description

SNX9: May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F- actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate. Belongs to the sorting nexin family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: ruffle; extrinsic to internal side of plasma membrane; cytoplasmic vesicle membrane; clathrin-coated vesicle; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; protein homodimerization activity; ubiquitin protein ligase binding; phosphoinositide binding; phosphatidylinositol binding

Biological Process: intracellular protein transport; mitosis; receptor-mediated endocytosis; positive regulation of protein oligomerization; cytokinesis after mitosis; vesicle organization and biogenesis; positive regulation of protein kinase activity; endocytosis; endosome transport; positive regulation of membrane protein ectodomain proteolysis; positive regulation of GTPase activity