Western Blot (WB)
(Anti-ASCL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:28 kDa)
Rabbit MASH1/ASCL1 Polyclonal Antibody | anti-ASCL1 antibody
Anti-MASH1/ASCL1 Antibody, Rabbit Polyclonal
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
IP: 1-5uL/mg of lysate
Western Blot (WB)
(Anti-ASCL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:28 kDa)
Western Blot (WB)
(Anti-ASCL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:28 kDa)
Immunoprecipitation (IP)
(CAV3 was immunoprecipitated using: Lane A:0.5 mg Mouse brains Tissue Lysate 4 uL anti-CAV3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAV3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size :29 kDa)
Immunoprecipitation (IP)
(CAV3 was immunoprecipitated using: Lane A:0.5 mg Mouse brains Tissue Lysate 4 uL anti-CAV3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAV3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size :29 kDa)
NCBI and Uniprot Product Information
Uniprot Description
ASCL1: Transcriptional regulator. May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcription activation by binding to the E box (5'-CANNTG-3').
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 10|10 C1
Cellular Component: cell soma; nucleus; RNA polymerase II transcription factor complex
Molecular Function: bHLH transcription factor binding; chromatin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; protein binding; protein dimerization activity; protein homodimerization activity; sequence-specific DNA binding
Biological Process: cell differentiation; cell maturation; central nervous system neuron development; cerebral cortex development; commitment of a neuronal cell to a specific type of neuron in the forebrain; enteric nervous system development; forebrain neuron differentiation; generation of neurons; glial cell differentiation; multicellular organism development; musculoskeletal movement, spinal reflex action; negative regulation of apoptosis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; neuroblast fate determination; neuroblast proliferation; neurogenesis; neuron development; neuron differentiation; neuron fate commitment; neuron fate specification; neuron migration; noradrenergic neuron development; noradrenergic neuron fate commitment; Notch signaling pathway; olfactory pit development; oligodendrocyte cell fate commitment; oligodendrocyte development; oligodendrocyte differentiation; parasympathetic nervous system development; pattern specification process; positive regulation of cell cycle; positive regulation of neural precursor cell proliferation; positive regulation of neurogenesis; positive regulation of neuron apoptotic process; positive regulation of neuron differentiation; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; regulation of epithelial cell differentiation; regulation of gene expression; regulation of mitotic cell cycle; regulation of neurogenesis; regulation of Notch signaling pathway; regulation of timing of subpallium neuron differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; spinal cord association neuron differentiation; spinal cord oligodendrocyte cell differentiation; spinal cord oligodendrocyte cell fate specification; subpallium neuron fate commitment; sympathetic nervous system development; transcription, DNA-dependent; vestibular nucleus development
Research Articles on ASCL1
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Product Notes
The ASCL1 ascl1 (Catalog #AAA8103422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MASH1/ASCL1 Antibody, Rabbit Polyclonal reacts with Mouse Predicted to React with: Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's MASH1/ASCL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the ASCL1 ascl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MASH1/ASCL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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