Human Sorting nexin-9 ELISA Kit | SNX9 elisa kit

Human Sorting nexin-9 ELISA Kit

Cat. Num. AAA765666

• Gene Names: SNX9; SDP1; WISP; SH3PX1; SH3PXD3A
• Reactivity: Human
• Synonyms: Sorting nexin-9; Human Sorting nexin-9 ELISA Kit; SNX9 (Sorting nexin-9)/SH3PX1/SH3PXD3A/MST155/MSTP155/SDP1/WISP/Protein SDP1/SH3 and PX domain-containing protein 1/SH3 and PX domain-containing protein 3A; SNX9 elisa kit

• Reactivity: Human
• Sequence Length: 595

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SNX9 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SNX9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SNX9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SNX9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SNX9 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7706706
• NCBI GeneID: 51429
• NCBI Accession #: NP_057308.1
• NCBI GenBank Nucleotide #: NM_016224.4
• Molecular Weight: 66,592 Da
• NCBI Official Full Name: sorting nexin-9
• NCBI Official Synonym Full Names: sorting nexin 9
• NCBI Official Symbol: SNX9
• NCBI Official Synonym Symbols: SDP1; WISP; SH3PX1; SH3PXD3A
• NCBI Protein Information: sorting nexin-9
• UniProt Protein Name: Sorting nexin-9
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX9
• UniProt Synonym Gene Names: SH3PX1; SH3PXD3A; Protein SDP1
• UniProt Entry Name: SNX9_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phosphoinositide binding domain, and are involved in intracellular trafficking. The encoded protein does not contain a coiled coil region, like some family members, but does contain a SRC homology domain near its N-terminus. The encoded protein is reported to have a variety of interaction partners, including of adaptor protein 2 , dynamin, tyrosine kinase non-receptor 2, Wiskott-Aldrich syndrome-like, and ARP3 actin-related protein 3. The encoded protein is implicated in several stages of intracellular trafficking, including endocytosis, macropinocytosis, and F-actin nucleation. [provided by RefSeq, Jul 2013]

Uniprot Description

SNX9: May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F- actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate. Belongs to the sorting nexin family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: clathrin-coated vesicle; cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasmic vesicle membrane; endosome; extrinsic to internal side of plasma membrane; plasma membrane; ruffle; trans-Golgi network

Molecular Function: phosphatidylinositol binding; phosphoinositide binding; protein binding; protein homodimerization activity; ubiquitin protein ligase binding

Biological Process: cytokinesis after mitosis; endocytosis; endosome transport; intracellular protein transport; mitosis; positive regulation of GTPase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of protein kinase activity; positive regulation of protein oligomerization; receptor-mediated endocytosis; vesicle organization and biogenesis