MAPK8IP3 sirna

MAPK8IP3 siRNA (Mouse)

Cat. Num. AAA8205869

• Gene Names: Mapk8ip3; Jip3; Syd2; JIP-3; JSAP1; JSAP1a; JSAP1b; JSAP1c; JSAP1d; BB120594; D17Wsu15e; mKIAA1066
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAPK8IP3; MAPK8IP3 siRNA (Mouse); JIP3; JSAP1; SYD2; C-Jun-amino-terminal kinase-interacting protein 3; JIP-3; JNK-interacting protein 3; JNK MAP kinase scaffold protein 3; JNK/SAPK-associated protein 1; Mitogen-activated protein kinase 8-interacting protein 3; Sunday driver 2; MAPK8IP3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAPK8IP3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1336

• Applicable Applications for MAPK8IP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAPK8IP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAPK8IP3 sirna

siRNA to inhibit MAPK8IP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 254540198
• NCBI GeneID: 30957
• NCBI Accession #: NP_001156919.1
• NCBI GenBank Nucleotide #: NM_001163447.1
• UniProt Accession #: Q9ESN9; Q5D062; Q99KU7; Q9EQD8; Q9ESN7; Q9ESN8; Q9ESP0; Q9JLH2; Q9JLH3; Q9R0U7
• Molecular Weight: 148,587 Da
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 3 isoform b
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 3
• NCBI Official Symbol: Mapk8ip3
• NCBI Official Synonym Symbols: Jip3; Syd2; JIP-3; JSAP1; JSAP1a; JSAP1b; JSAP1c; JSAP1d; BB120594; D17Wsu15e; mKIAA1066
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 3
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 3
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: Mapk8ip3
• UniProt Synonym Gene Names: Jip3; Jsap1; Syd2; JIP-3; JNK-interacting protein 3; JSAP1
• UniProt Entry Name: JIP3_MOUSE

Uniprot Description

JIP3: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies in the mouse suggested that this protein may interact with and regulate the activity of numerous protein kinases of the JNK signaling pathway. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: Golgi membrane; Golgi apparatus; neuron projection; cell projection; smooth endoplasmic reticulum; dendrite; cytoplasm; plasma membrane; cytoplasmic vesicle; axolemma

Molecular Function: JUN kinase binding; protein binding; MAP-kinase scaffold activity; receptor signaling complex scaffold activity; kinesin binding; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding

Biological Process: vesicle-mediated transport; axon guidance; positive regulation of JNK activity; protein localization; positive regulation of signal transduction; regulation of gene expression; in utero embryonic development; forebrain development; JNK cascade; respiratory gaseous exchange; positive regulation of neuron differentiation; alveolus development; regulation of JNK cascade; activation of JNK activity; post-embryonic development