MRE11A sirna

MRE11A siRNA (Rat)

Cat. Num. AAA8202825

• Gene Names: Mre11a; Mre11
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MRE11A; MRE11A siRNA (Rat); MRE11; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A; MRE11A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MRE11A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 706

• Applicable Applications for MRE11A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MRE11A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MRE11A sirna

siRNA to inhibit MRE11A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11560107
• NCBI GeneID: 64046
• NCBI Accession #: NP_071615.1
• NCBI GenBank Nucleotide #: NM_022279.1
• UniProt Accession #: Q9JIM0
• Molecular Weight: 80,122 Da
• NCBI Official Full Name: double-strand break repair protein MRE11A
• NCBI Official Synonym Full Names: MRE11 homolog A, double strand break repair nuclease
• NCBI Official Symbol: Mre11a
• NCBI Official Synonym Symbols: Mre11
• NCBI Protein Information: double-strand break repair protein MRE11A
• UniProt Protein Name: Double-strand break repair protein MRE11A
• Protein Family: Double-strand break repair protein
• UniProt Gene Name: Mre11a
• UniProt Synonym Gene Names: Mre11; MRE11 homolog 1; MRE11 homolog A
• UniProt Entry Name: MRE11_RAT

NCBI Description

may play a role in regulation of cardiomyocyte proliferation [RGD, Feb 2006]

Uniprot Description

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: Deoxyribonuclease; DNA repair, damage; Cell cycle regulation; DNA-binding

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; condensed nuclear chromosome; perinuclear region of cytoplasm; nuclear chromatin; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; manganese ion binding; endonuclease activity; exonuclease activity; 3'-5' exonuclease activity

Biological Process: negative regulation of virion penetration into host cell; heart development; positive regulation of kinase activity; sister chromatid cohesion; negative regulation of DNA endoreduplication; chromosome organization and biogenesis; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; cell proliferation; meiotic cell cycle; nucleotide-excision repair; base-excision repair; intra-S DNA damage checkpoint; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; synapsis; telomere maintenance; response to DNA damage stimulus